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BAG3 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant BAG3.

  • WB - BAG3 Antibody (monoclonal) (M01) AT1262a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (88.99 KDa) .
  • WB - BAG3 Antibody (monoclonal) (M01) AT1262a
    Western Blot analysis of BAG3 expression in transfected 293T cell line by BAG3 monoclonal antibody (M01), clone 5A8.

    Lane 1: BAG3 transfected lysate (Predicted MW: 61.6 KDa).
    Lane 2: Non-transfected lysate.
  • E - BAG3 Antibody (monoclonal) (M01) AT1262a
    Detection limit for recombinant GST tagged BAG3 is approximately 0.03ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O95817
Other Accession BC014656
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names 5A8
Calculated MW 61595 Da
Additional Information
Gene ID 9531
Other Names BAG family molecular chaperone regulator 3, BAG-3, Bcl-2-associated athanogene 3, Bcl-2-binding protein Bis, Docking protein CAIR-1, BAG3, BIS
Target/Specificity BAG3 (AAH14656.1, 1 a.a. ~ 575 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsBAG3 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.


Variation at the NFATC2 Locus Increases the Risk of Thiazolinedinedione-Induced Edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) Study. Bailey SD, et al. Diabetes Care, 2010 Jul 13. PMID 20628086.IKK{gamma} protein is a target of BAG3 regulatory activity in human tumor growth. Ammirante M, et al. Proc Natl Acad Sci U S A, 2010 Apr 20. PMID 20368414.Characterization of BAG3 cleavage during apoptosis of pancreatic cancer cells. Wang HQ, et al. J Cell Physiol, 2010 Jul. PMID 20232307.The co-chaperone BAG3 interacts with the cytosolic chaperonin CCT: new hints for actin folding. Fontanella B, et al. Int J Biochem Cell Biol, 2010 May. PMID 20018251.Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip. Talmud PJ, et al. Am J Hum Genet, 2009 Nov. PMID 19913121.

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Cat# AT1262a
(40 western blots)
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