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BUB1B Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant BUB1B.

  • WB - BUB1B Antibody (monoclonal) (M01) AT1320a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (39.93 KDa) .
  • WB - BUB1B Antibody (monoclonal) (M01) AT1320a
    BUB1B monoclonal antibody (M01), clone 2G9. Western Blot analysis of BUB1B expression in human spleen.
  • IHC - BUB1B Antibody (monoclonal) (M01) AT1320a
    Immunoperoxidase of monoclonal antibody to BUB1B on formalin-fixed paraffin-embedded human spleen. [antibody concentration 1 ug/ml]
  • IF - BUB1B Antibody (monoclonal) (M01) AT1320a
    Immunofluorescence of monoclonal antibody to BUB1B on HeLa cell. [antibody concentration 10 ug/ml]
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O60566
Other Accession BC018739
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 2G9
Calculated MW 119545 Da
Additional Information
Gene ID 701
Other Names Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, MAD3/BUB1-related protein kinase, hBUBR1, Mitotic checkpoint kinase MAD3L, Protein SSK1, BUB1B, BUBR1, MAD3L, SSK1
Target/Specificity BUB1B (AAH18739, 1 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsBUB1B Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.


Molecular causes for BUBR1 dysfunction in the human cancer predisposition syndrome mosaic variegated aneuploidy. Suijkerbuijk SJ, et al. Cancer Res, 2010 Jun 15. PMID 20516114.Spindle proteins in resected pancreatic head adenocarcinomas: BubR1 is an independent prognostic factor in pancreatobiliary-type tumours. Gladhaug IP, et al. Histopathology, 2010 Feb. PMID 20459534.A Large-scale genetic association study of esophageal adenocarcinoma risk. Liu CY, et al. Carcinogenesis, 2010 Jul. PMID 20453000.BUBR1 expression in benign oral lesions and squamous cell carcinomas: correlation with human papillomavirus. Lira RC, et al. Oncol Rep, 2010 Apr. PMID 20204288.LSD1 is required for chromosome segregation during mitosis. Lv S, et al. Eur J Cell Biol, 2010 Jul. PMID 20189264.

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$ 315.00
Cat# AT1320a
(40 western blots)
Availability: 7-10 days
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