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CA3 Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a full-length recombinant CA3.

     
  • WB - CA3 Antibody (monoclonal) (M02) AT1357a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (54.34 KDa) .
    detail
  • WB - CA3 Antibody (monoclonal) (M02) AT1357a
    CA3 monoclonal antibody (M02), clone 4A12-1A3 Western Blot analysis of CA3 expression in K-562 ( (Cat # AT1357a )
    detail
  • IHC - CA3 Antibody (monoclonal) (M02) AT1357a
    Immunoperoxidase of monoclonal antibody to CA3 on formalin-fixed paraffin-embedded human skeletal muscle. [antibody concentration 3 ug/ml]
    detail
  • E - CA3 Antibody (monoclonal) (M02) AT1357a
    Detection limit for recombinant GST tagged CA3 is approximately 1ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession P07451
Other Accession BC004897
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 4A12-1A3
Calculated MW 29557 Da
Additional Information
Gene ID 761
Other Names Carbonic anhydrase 3, Carbonate dehydratase III, Carbonic anhydrase III, CA-III, CA3
Target/Specificity CA3 (AAH04897, 1 a.a. ~ 260 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsCA3 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns.

References

1.Proteomic profiling of antisense-induced exon skipping reveals reversal of pathobiochemical abnormalities in dystrophic mdx diaphragm.Doran P, Wilton SD, Fletcher S, Ohlendieck K.Proteomics. 2009 Feb;9(3):671-85.

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$ 350.00
Cat# AT1357a
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