CALML5 Antibody (monoclonal) (M16)
Mouse monoclonal antibody raised against a full-length recombinant CALML5.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, E |
---|---|
Primary Accession | Q9NZT1 |
Other Accession | BC039172 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Isotype | IgG2a Kappa |
Clone Names | 2F10 |
Calculated MW | 15893 Da |
Gene ID | 51806 |
---|---|
Other Names | Calmodulin-like protein 5, Calmodulin-like skin protein, CALML5, CLSP |
Target/Specificity | CALML5 (AAH39172, 1 a.a. ~ 146 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Dilution | WB~~1:500~1000 |
Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
Precautions | CALML5 Antibody (monoclonal) (M16) is for research use only and not for use in diagnostic or therapeutic procedures. |
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Background
This gene encodes a novel calcium binding protein expressed in the epidermis and related to the calmodulin family of calcium binding proteins. Functional studies with recombinant protein demonstrate it does bind calcium and undergoes a conformational change when it does so. Abundant expression is detected only in reconstructed epidermis and is restricted to differentiating keratinocytes. In addition, it can associate with transglutaminase 3, shown to be a key enzyme in the terminal differentiation of keratinocytes.
References
Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931.A structural and dynamic characterization of the EF-hand protein CLSP. Babini E, et al. Structure, 2006 Jun. PMID 16765896.Influence of calcium on the proteolytic degradation of the calmodulin-like skin protein (calmodulin-like protein 5) in psoriatic epidermis. M?hul B, et al. Exp Dermatol, 2006 Jun. PMID 16689864.The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.
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