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>   home   >   Products   >   Primary Antibodies   >   CBX5 Antibody (monoclonal) (M01)   

CBX5 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant CBX5.

  • WB - CBX5 Antibody (monoclonal) (M01) AT1411a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (46.75 KDa) .
  • WB - CBX5 Antibody (monoclonal) (M01) AT1411a
    CBX5 monoclonal antibody (M01), clone 1E11-3A10 Western Blot analysis of CBX5 expression in Hela S3 NE ( (Cat # AT1411a )
  • WB - CBX5 Antibody (monoclonal) (M01) AT1411a
    Western Blot analysis of CBX5 expression in transfected 293T cell line by CBX5 monoclonal antibody (M01), clone 1E11-3A10.

    Lane 1: CBX5 transfected lysate(22.2 KDa).
    Lane 2: Non-transfected lysate.
  • IF - CBX5 Antibody (monoclonal) (M01) AT1411a
    Immunofluorescence of monoclonal antibody to CBX5 on HeLa cell. [antibody concentration 10 ug/ml]
  • E - CBX5 Antibody (monoclonal) (M01) AT1411a
    Detection limit for recombinant GST tagged CBX5 is approximately 0.03ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P45973
Other Accession BC006821
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 1E11-3A10
Calculated MW 22225 Da
Additional Information
Gene ID 23468
Other Names Chromobox protein homolog 5, Antigen p25, Heterochromatin protein 1 homolog alpha, HP1 alpha, CBX5, HP1A
Target/Specificity CBX5 (AAH06821, 1 a.a. ~ 191 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsCBX5 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.


Human POGZ modulates dissociation of HP1alpha from mitotic chromosome arms through Aurora B activation. Nozawa RS, et al. Nat Cell Biol, 2010 Jul. PMID 20562864.Lamin A rod domain mutants target heterochromatin protein 1alpha and beta for proteasomal degradation by activation of F-box protein, FBXW10. Chaturvedi P, et al. PLoS One, 2010 May 13. PMID 20498703.Inner centromere formation requires hMis14, a trident kinetochore protein that specifically recruits HP1 to human chromosomes. Kiyomitsu T, et al. J Cell Biol, 2010 Mar 22. PMID 20231385.Protein complex of Drosophila ATRX/XNP and HP1a is required for the formation of pericentric beta-heterochromatin in vivo. Emelyanov AV, et al. J Biol Chem, 2010 May 14. PMID 20154359.ATRX interacts with H3.3 in maintaining telomere structural integrity in pluripotent embryonic stem cells. Wong LH, et al. Genome Res, 2010 Mar. PMID 20110566.

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$ 315.00
Cat# AT1411a
(40 western blots)
Availability: 7-10 days
Bulk Size
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