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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   CDC25C Antibody (monoclonal) (M01)   

CDC25C Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant CDC25C.

  • WB - CDC25C Antibody (monoclonal) (M01) AT1458a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.73 KDa) .
  • WB - CDC25C Antibody (monoclonal) (M01) AT1458a
    CDC25C monoclonal antibody (M01), clone 3B11 Western Blot analysis of CDC25C expression in HeLa ( (Cat # AT1458a )
  • WB - CDC25C Antibody (monoclonal) (M01) AT1458a
    Western Blot analysis of CDC25C expression in transfected 293T cell line by CDC25C monoclonal antibody (M01), clone 3B11.

    Lane 1: CDC25C transfected lysate(53.312 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - CDC25C Antibody (monoclonal) (M01) AT1458a
    Immunoperoxidase of monoclonal antibody to CDC25C on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 6 ug/ml]
  • IP - CDC25C Antibody (monoclonal) (M01) AT1458a
    Immunoprecipitation of CDC25C transfected lysate using anti-CDC25C monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with CDC25C MaxPab rabbit polyclonal antibody.
  • E - CDC25C Antibody (monoclonal) (M01) AT1458a
    Detection limit for recombinant GST tagged CDC25C is approximately 0.1ng/ml as a capture antibody.
  • WB - CDC25C Antibody (monoclonal) (M01) AT1458a
    Western blot analysis of CDC25C over-expressed 293 cell line, cotransfected with CDC25C Validated Chimera RNAi ( (Cat # AT1458a )
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P30307
Other Accession BC019089
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 3B11
Calculated MW 53365 Da
Additional Information
Gene ID 995
Other Names M-phase inducer phosphatase 3, Dual specificity phosphatase Cdc25C, CDC25C
Target/Specificity CDC25C (AAH19089, 21 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsCDC25C Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known.


Centrosome-related genes, genetic variation, and risk of breast cancer. Olson JE, et al. Breast Cancer Res Treat, 2010 May 28. PMID 20508983.Overexpression of CDC25B, CDC25C and phospho-CDC25C (Ser216) in vulvar squamous cell carcinomas are associated with malignant features and aggressive cancer phenotypes. Wang Z, et al. BMC Cancer, 2010 May 25. PMID 20500813.A Large-scale genetic association study of esophageal adenocarcinoma risk. Liu CY, et al. Carcinogenesis, 2010 Jul. PMID 20453000.Cdc25 phosphatases are required for timely assembly of CDK1-cyclin B at the G2/M transition. Timofeev O, et al. J Biol Chem, 2010 May 28. PMID 20360007.Adventitious arsenate reductase activity of the catalytic domain of the human Cdc25B and Cdc25C phosphatases. Bhattacharjee H, et al. Biochemistry, 2010 Feb 2. PMID 20025242.

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$ 315.00
Cat# AT1458a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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