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CFL2 Antibody (monoclonal) (M03)

Mouse monoclonal antibody raised against a partial recombinant CFL2.

  • WB - CFL2 Antibody (monoclonal) (M03) AT1507a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.84 KDa) .
  • WB - CFL2 Antibody (monoclonal) (M03) AT1507a
    CFL2 monoclonal antibody (M03), clone 6G9 Western Blot analysis of CFL2 expression in HeLa ( (Cat # AT1507a )
  • IHC - CFL2 Antibody (monoclonal) (M03) AT1507a
    Immunoperoxidase of monoclonal antibody to CFL2 on formalin-fixed paraffin-embedded human skeletal muscle. [antibody concentration 8 ug/ml]
  • IF - CFL2 Antibody (monoclonal) (M03) AT1507a
    Immunofluorescence of monoclonal antibody to CFL2 on HeLa cell. [antibody concentration 10 ug/ml]
  • E - CFL2 Antibody (monoclonal) (M03) AT1507a
    Detection limit for recombinant GST tagged CFL2 is approximately 1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y281
Other Accession NM_021914
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 6G9
Calculated MW 18737 Da
Additional Information
Gene ID 1073
Other Names Cofilin-2, Cofilin, muscle isoform, CFL2
Target/Specificity CFL2 (NP_068733, 57 a.a. ~ 166 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsCFL2 Antibody (monoclonal) (M03) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. This protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants.


Muscle LIM protein interacts with cofilin 2 and regulates F-actin dynamics in cardiac and skeletal muscle. Papalouka V, et al. Mol Cell Biol, 2009 Nov. PMID 19752190.Cofilin activation in peripheral CD4 T cells of HIV-1 infected patients: a pilot study. Wu Y, et al. Retrovirology, 2008 Oct 17. PMID 18928553.Nemaline myopathy with minicores caused by mutation of the CFL2 gene encoding the skeletal muscle actin-binding protein, cofilin-2. Agrawal PB, et al. Am J Hum Genet, 2007 Jan. PMID 17160903.Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Olsen JV, et al. Cell, 2006 Nov 3. PMID 17081983.Cofilin cross-bridges adjacent actin protomers and replaces part of the longitudinal F-actin interface. Kudryashov DS, et al. J Mol Biol, 2006 May 5. PMID 16530787.

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$ 315.00
Cat# AT1507a
(40 western blots)
Availability: 7-10 days
Bulk Size
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