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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   CHFR Antibody (monoclonal) (M08)   

CHFR Antibody (monoclonal) (M08)

Mouse monoclonal antibody raised against a partial recombinant CHFR.

  • WB - CHFR Antibody (monoclonal) (M08) AT1519a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (32.12 KDa) .
  • WB - CHFR Antibody (monoclonal) (M08) AT1519a
    Western Blot analysis of CHFR expression in transfected 293T cell line by CHFR monoclonal antibody (M08), clone 2B11.

    Lane 1: CHFR transfected lysate(72.1 KDa).
    Lane 2: Non-transfected lysate.
  • E - CHFR Antibody (monoclonal) (M08) AT1519a
    Detection limit for recombinant GST tagged CHFR is 0.03 ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q96EP1
Other Accession BC012072
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 2B11
Calculated MW 73386 Da
Additional Information
Gene ID 55743
Other Names E3 ubiquitin-protein ligase CHFR, 632-, Checkpoint with forkhead and RING finger domains protein, RING finger protein 196, CHFR, RNF196
Target/Specificity CHFR (AAH12072.1, 136 a.a. ~ 194 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsCHFR Antibody (monoclonal) (M08) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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CHFR functions as a ubiquitin ligase for HLTF to regulate its stability and functions. Kim JM, et al. Biochem Biophys Res Commun, 2010 May 14. PMID 20388495.Aberrant gene methylation in the peritoneal fluid is a risk factor predicting peritoneal recurrence in gastric cancer. Hiraki M, et al. World J Gastroenterol, 2010 Jan 21. PMID 20082478.Aberrant promoter hypermethylation of the CHFR gene in oral squamous cell carcinomas. Baba S, et al. Oncol Rep, 2009 Nov. PMID 19787237.CHFR promoter hypermethylation and reduced CHFR mRNA expression in ovarian cancer. Gao Y, et al. Int J Biol Markers, 2009 Apr-Jun. PMID 19634111.Novel susceptibility loci for second primary tumors/recurrence in head and neck cancer patients: large-scale evaluation of genetic variants. Wu X, et al. Cancer Prev Res (Phila), 2009 Jul. PMID 19584075.

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$ 315.00
Cat# AT1519a
(40 western blots)
Availability: 7-10 days
Bulk Size
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