COX4I2 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant COX4I2.
|Application ||WB, IHC, IP, E|
|Calculated MW||20010 Da|
|Other Names||Cytochrome c oxidase subunit 4 isoform 2, mitochondrial, Cytochrome c oxidase subunit IV isoform 2, COX IV-2, COX4I2, COX4L2|
|Target/Specificity||COX4I2 (NP_115998, 21 a.a. ~ 104 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Format||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.|
|Precautions||COX4I2 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes isoform 2 of subunit IV. Isoform 1 of subunit IV is encoded by a different gene, however, the two genes show a similar structural organization. Subunit IV is the largest nuclear encoded subunit which plays a pivotal role in COX regulation.
1.Oxygen-dependent expression of cytochrome c oxidase subunit 4-2 gene expression is mediated by transcription factors RBPJ, CXXC5 and CHCHD2.Aras S, Pak O, Sommer N, Finley R Jr, Huttemann M, Weissmann N, Grossman LI.Nucleic Acids Res. 2013 Jan 8.2.Cytochrome c oxidase subunit 4 isoform 2-knockout mice show reduced enzyme activity, airway hyporeactivity, and lung pathology.Huttemann M, Lee I, Gao X, Pecina P, Pecinova A, Liu J, Aras S, Sommer N, Sanderson TH, Tost M, Neff F, Aguilar-Pimentel JA, Becker L, Naton B, Rathkolb B, Rozman J, Favor J, Hans W, Prehn C, Puk O, Schrewe A, Sun M, Hofler H, Adamski J, Bekeredjian R, Graw J, Adler T, Busch DH, Klingenspor M, Klopstock T, Ollert M, Wolf E, Fuchs H, Gailus-Durner V, Hrabe de Angelis M, Weissmann N, Doan JW, Bassett DJ, Grossman LI.FASEB J. 2012 Sep;26(9):3916-30. Epub 2012 Jun 22.3.Sex- and brain region-specific role of cytochrome c oxidase in 1-methyl-4-phenylpyridinium-mediated astrocyte vulnerability.Sundar Boyalla S, Barbara Victor M, Roemgens A, Beyer C, Arnold S.J Neurosci Res. 2011 Dec;89(12):2068-82. doi: 10.1002/ jnr.22669. Epub 2011 May 19.4.Brain region-specific vulnerability of astrocytes in response to 3-nitropropionic acid is mediated by cytochrome c oxidase isoform expression.Misiak M, Singh S, Drewlo S, Beyer C, Arnold S.Cell Tissue Res. 2010 Jul;341(1):83-93. Epub 2010 Jul 3.5.Brain region specificity of 3-nitropropionic acid-induced vulnerability of neurons involves cytochrome c oxidase.Singh S, Misiak M, Beyer C, Arnold S.Neurochem Int. 2010 Jun 23. [Epub ahead of print]
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