CPSF3 Antibody (monoclonal) (M06)
Mouse monoclonal antibody raised against a partial recombinant CPSF3.
|Reactivity||Human, Mouse, Rat|
|Calculated MW||77486 Da|
|Other Names||Cleavage and polyadenylation specificity factor subunit 3, 3127-, Cleavage and polyadenylation specificity factor 73 kDa subunit, CPSF 73 kDa subunit, mRNA 3'-end-processing endonuclease CPSF-73, CPSF3, CPSF73|
|Target/Specificity||CPSF3 (NP_057291, 585 a.a. ~ 684 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Format||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.|
|Precautions||CPSF3 Antibody (monoclonal) (M06) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Most eukaryotic mRNA precursors (pre-mRNAs) undergo extensive maturational processing, including cleavage and polyadenylation at the 3-prime end. CPSF3 encodes the 73-kD subunit of the cleavage and polyadenylation specificity factor (CPSF) and is the pre-mRNA 3-prime-end-processing endonuclease (Mandel et al., 2006 [PubMed 17128255]).
The tumor suppressor Cdc73 functionally associates with CPSF and CstF 3' mRNA processing factors. Rozenblatt-Rosen O, et al. Proc Natl Acad Sci U S A, 2009 Jan 20. PMID 19136632.Studies of the 5' exonuclease and endonuclease activities of CPSF-73 in histone pre-mRNA processing. Yang XC, et al. Mol Cell Biol, 2009 Jan. PMID 18955505.CSR1 induces cell death through inactivation of CPSF3. Zhu ZH, et al. Oncogene, 2009 Jan 8. PMID 18806823.Conserved motifs in both CPSF73 and CPSF100 are required to assemble the active endonuclease for histone mRNA 3'-end maturation. Kolev NG, et al. EMBO Rep, 2008 Oct. PMID 18688255.The 73 kDa subunit of the CPSF complex binds to the HIV-1 LTR promoter and functions as a negative regulatory factor that is inhibited by the HIV-1 Tat protein. de la Vega L, et al. J Mol Biol, 2007 Sep 14. PMID 17669424.
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