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CRLF1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant CRLF1.

  • WB - CRLF1 Antibody (monoclonal) (M01) AT1626a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.3 KDa) .
  • WB - CRLF1 Antibody (monoclonal) (M01) AT1626a
    CRLF1 monoclonal antibody (M01), clone 4F4 Western Blot analysis of CRLF1 expression in HeLa ( (Cat # AT1626a )
  • WB - CRLF1 Antibody (monoclonal) (M01) AT1626a
    Western Blot analysis of CRLF1 expression in transfected 293T cell line by CRLF1 monoclonal antibody (M01), clone 4F4.

    Lane 1: CRLF1 transfected lysate(46.3 KDa).
    Lane 2: Non-transfected lysate.
  • IP - CRLF1 Antibody (monoclonal) (M01) AT1626a
    Immunoprecipitation of CRLF1 transfected lysate using anti-CRLF1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with CRLF1 MaxPab rabbit polyclonal antibody.
  • E - CRLF1 Antibody (monoclonal) (M01) AT1626a
    Detection limit for recombinant GST tagged CRLF1 is approximately 0.03ng/ml as a capture antibody.
  • WB - CRLF1 Antibody (monoclonal) (M01) AT1626a
    Western blot analysis of CRLF1 over-expressed 293 cell line, cotransfected with CRLF1 Validated Chimera RNAi ( (Cat # AT1626a )
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O75462
Other Accession NM_004750
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 4F4
Calculated MW 46302 Da
Additional Information
Gene ID 9244
Other Names Cytokine receptor-like factor 1, Cytokine-like factor 1, CLF-1, ZcytoR5, CRLF1
Target/Specificity CRLF1 (NP_004741, 135 a.a. ~ 230 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsCRLF1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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This gene encodes a member of the cytokine type I receptor family. The protein forms a secreted complex with cardiotrophin-like cytokine factor 1 and acts on cells expressing ciliary neurotrophic factor receptors. The complex can promote survival of neuronal cells. Mutations in this gene result in Crisponi syndrome and cold-induced sweating syndrome.


Cytokine receptor-like factor 1 is highly expressed in damaged human knee osteoarthritic cartilage and involved in osteoarthritis downstream of TGF-beta. Tsuritani K, et al. Calcif Tissue Int, 2010 Jan. PMID 19921088.Crisponi syndrome is caused by mutations in the CRLF1 gene and is allelic to cold-induced sweating syndrome type 1. Crisponi L, et al. Am J Hum Genet, 2007 May. PMID 17436252.Mutations in cytokine receptor-like factor 1 (CRLF1) account for both Crisponi and cold-induced sweating syndromes. Dagoneau N, et al. Am J Hum Genet, 2007 May. PMID 17436251.The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.Signal peptide prediction based on analysis of experimentally verified cleavage sites. Zhang Z, et al. Protein Sci, 2004 Oct. PMID 15340161.

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$ 315.00
Cat# AT1626a
(40 western blots)
Availability: 7-10 days
Bulk Size
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