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CTSK Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant CTSK.

     
  • WB - CTSK Antibody (monoclonal) (M01) AT1681a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.95 KDa) .
    detail
  • WB - CTSK Antibody (monoclonal) (M01) AT1681a
    Western Blot analysis of CTSK expression in transfected 293T cell line by CTSK monoclonal antibody (M01), clone 2F1.

    Lane 1: CTSK transfected lysate(37 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • IHC - CTSK Antibody (monoclonal) (M01) AT1681a
    Immunoperoxidase of monoclonal antibody to CTSK on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]
    detail
  • E - CTSK Antibody (monoclonal) (M01) AT1681a
    Detection limit for recombinant GST tagged CTSK is approximately 3ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession P43235
Other Accession BC016058
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 2F1
Calculated MW 36966 Da
Additional Information
Gene ID 1513
Other Names Cathepsin K, Cathepsin O, Cathepsin O2, Cathepsin X, CTSK, CTSO, CTSO2
Target/Specificity CTSK (AAH16058, 220 a.a. ~ 329 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsCTSK Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. This gene may be subject to RNA editing.

References

1.Simultaneous expression of Cathepsins B and K in pulmonary adenocarcinomas and squamous cell carcinomas predicts poor recurrence-free and overall survival.Cordes C, Bartling B, Simm A, Afar D, Lautenschlager C, Hansen G, Silber RE, Burdach S, Hofmann HS.Lung Cancer. 2009 Apr;64(1):79-85. Epub 2008 Aug 29.

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$ 350.00
Cat# AT1681a
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