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CYP2J2 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant CYP2J2.

     
  • WB - CYP2J2 Antibody (monoclonal) (M01) AT1704a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (35.75 KDa) .
    detail
  • E - CYP2J2 Antibody (monoclonal) (M01) AT1704a
    Detection limit for recombinant GST tagged CYP2J2 is approximately 3ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P51589
Other Accession NM_000775
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 2D10
Calculated MW 57611 Da
Additional Information
Gene ID 1573
Other Names Cytochrome P450 2J2, Arachidonic acid epoxygenase, CYPIIJ2, CYP2J2
Target/Specificity CYP2J2 (NP_000766, 408 a.a. ~ 498 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsCYP2J2 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is thought to be the predominant enzyme responsible for epoxidation of endogenous arachidonic acid in cardiac tissue.

References

1.CYP2J2 Expression and Circulating Epoxyeicosatrienoic Metabolites in Preeclampsia.Herse F, Lamarca B, Hubel CA, Kaartokallio T, Lokki AI, Ekholm E, Laivuori H, Gauster M, Huppertz B, Sugulle M, Ryan MJ, Novotny S, Brewer J, Park JK, Kacik M, Hoyer J, Verlohren S, Wallukat G, Rothe M, Luft FC, Muller DN, Schunck WH, Staff AC, Dechend R.Circulation. 2012 Dec 18;126(25):2990-9. doi: 10.1161/CIRCULATIONAHA.112.127340. Epub 2012 Nov 15.2.Identifying a Selective Substrate and Inhibitor Pair for the Evaluation of CYP2J2 Activity.Lee CA, Jones JP 3rd, Katayama J, Kaspera R, Jiang Y, Freiwald S, Smith E, Walker GS, Totah RA.Drug Metab Dispos. 2012 May;40(5):943-51. Epub 2012 Feb 10.3.Detection of EETs and HETEs-generating Cytochrome P450 Enzymes and the Effects of their Metabolites on Myometrial and Vascular Function.Pearson T, Warren AY, Barrett DA, Khan RN.Am J Physiol Endocrinol Metab. 2009 Sep;297(3):E647-56. Epub 2009 Jun 23.

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$ 350.00
Cat# AT1704a
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Availability: 7-10 days
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