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CYP7B1 Antibody (monoclonal) (M06)

Mouse monoclonal antibody raised against a partial recombinant CYP7B1.

  • WB - CYP7B1 Antibody (monoclonal) (M06) AT1706a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (34.98 KDa) .
  • WB - CYP7B1 Antibody (monoclonal) (M06) AT1706a
    Western Blot analysis of CYP7B1 expression in transfected 293T cell line by CYP7B1 monoclonal antibody (M06), clone 2B11.

    Lane 1: CYP7B1 transfected lysate(58.256 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - CYP7B1 Antibody (monoclonal) (M06) AT1706a
    Immunoperoxidase of monoclonal antibody to CYP7B1 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml]
  • E - CYP7B1 Antibody (monoclonal) (M06) AT1706a
    Detection limit for recombinant GST tagged CYP7B1 is approximately 0.3ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O75881
Other Accession NM_004820
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 2B11
Calculated MW 58256 Da
Additional Information
Gene ID 9420
Other Names 25-hydroxycholesterol 7-alpha-hydroxylase, Cytochrome P450 7B1, Oxysterol 7-alpha-hydroxylase, CYP7B1
Target/Specificity CYP7B1 (NP_004811, 203 a.a. ~ 286 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsCYP7B1 Antibody (monoclonal) (M06) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis.


Variation at the NFATC2 Locus Increases the Risk of Thiazolinedinedione-Induced Edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) Study. Bailey SD, et al. Diabetes Care, 2010 Jul 13. PMID 20628086.Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip. Talmud PJ, et al. Am J Hum Genet, 2009 Nov. PMID 19913121.CYP7B1-mediated metabolism of 5alpha-androstane-3alpha,17beta-diol (3alpha-Adiol): a novel pathway for potential regulation of the cellular levels of androgens and neurosteroids. Pettersson H, et al. Biochim Biophys Acta, 2009 Dec. PMID 19732851.Genetic susceptibility to distinct bladder cancer subphenotypes. Guey LT, et al. Eur Urol, 2010 Feb. PMID 19692168.CYP7B1: one cytochrome P450, two human genetic diseases, and multiple physiological functions. Stiles AR, et al. J Biol Chem, 2009 Oct 16. PMID 19687010.

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$ 315.00
Cat# AT1706a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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