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DDEF1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant DDEF1.

     
  • IF - DDEF1 Antibody (monoclonal) (M01) AT1729a
    Immunofluorescence of monoclonal antibody to DDEF1 on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - DDEF1 Antibody (monoclonal) (M01) AT1729a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
    detail
  • WB - DDEF1 Antibody (monoclonal) (M01) AT1729a
    DDEF1 monoclonal antibody (M01), clone 2G7. Western Blot analysis of DDEF1 expression in PC-12 ( (Cat # AT1729a )
    detail
  • WB - DDEF1 Antibody (monoclonal) (M01) AT1729a
    DDEF1 monoclonal antibody (M01), clone 2G7 Western Blot analysis of DDEF1 expression in IMR-32 ( (Cat # AT1729a )
    detail
  • E - DDEF1 Antibody (monoclonal) (M01) AT1729a
    Detection limit for recombinant GST tagged DDEF1 is approximately 0.03ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession Q9ULH1
Other Accession NM_018482
Reactivity Human, Rat
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 2G7
Calculated MW 125498 Da
Additional Information
Gene ID 50807
Other Names Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1, 130 kDa phosphatidylinositol 4, 5-bisphosphate-dependent ARF1 GTPase-activating protein, ADP-ribosylation factor-directed GTPase-activating protein 1, ARF GTPase-activating protein 1, Development and differentiation-enhancing factor 1, DEF-1, Differentiation-enhancing factor 1, PIP2-dependent ARF1 GAP, ASAP1, DDEF1, KIAA1249
Target/Specificity DDEF1 (NP_060952, 1030 a.a. ~ 1129 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsDDEF1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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References

ASAP1 promotes tumor cell motility and invasiveness, stimulates metastasis formation in vivo, and correlates with poor survival in colorectal cancer patients. M?ller T, et al. Oncogene, 2010 Apr 22. PMID 20154719.Genome-wide association study identifies new multiple sclerosis susceptibility loci on chromosomes 12 and 20. Australia and New Zealand Multiple Sclerosis Genetics Consortium (ANZgene). Nat Genet, 2009 Jul. PMID 19525955.Identification of interaction partners for individual SH3 domains of Fas ligand associated members of the PCH protein family in T lymphocytes. Linkermann A, et al. Biochim Biophys Acta, 2009 Feb. PMID 19041431.Autoinhibition of Arf GTPase-activating protein activity by the BAR domain in ASAP1. Jian X, et al. J Biol Chem, 2009 Jan 16. PMID 19017632.Arf GTPase-activating protein ASAP1 interacts with Rab11 effector FIP3 and regulates pericentrosomal localization of transferrin receptor-positive recycling endosome. Inoue H, et al. Mol Biol Cell, 2008 Oct. PMID 18685082.

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$ 350.00
Cat# AT1729a
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