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DEF6 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant DEF6.

     
  • IP - DEF6 Antibody (monoclonal) (M01) AT1749a
    Immunoprecipitation of DEF6 transfected lysate using anti-DEF6 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with DEF6 monoclonal antibody.
    detail
  • WB - DEF6 Antibody (monoclonal) (M01) AT1749a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (62.7 KDa) .
    detail
  • IHC - DEF6 Antibody (monoclonal) (M01) AT1749a
    Immunoperoxidase of monoclonal antibody to DEF6 on formalin-fixed paraffin-embedded human lymph node [antibody concentration 3 ug/ml]
    detail
  • E - DEF6 Antibody (monoclonal) (M01) AT1749a
    Detection limit for recombinant GST tagged DEF6 is approximately 1ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IP, E
Primary Accession Q9H4E7
Other Accession BC017504
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1F2
Calculated MW 73910 Da
Additional Information
Gene ID 50619
Other Names Differentially expressed in FDCP 6 homolog, DEF-6, IRF4-binding protein, DEF6, IBP
Target/Specificity DEF6 (AAH17504, 1 a.a. ~ 336 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsDEF6 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

DEF6, or IBP, is a guanine nucleotide exchange factor (GEF) for RAC (MIM 602048) and CDC42 (MIM 116952) that is highly expressed in B and T cells (Gupta et al., 2003 [PubMed 12923183]).[supplied by OMIM]

References

1.Def-6, a Novel Regulator of Small GTPases in Podocytes, Acts Downstream of Atypical Protein Kinase C (aPKC) ?/?.Worthmann K, Leitges M, Teng B, Sestu M, Tossidou I, Samson T, Haller H, Huber TB, Schiffer MAm J Pathol. 2013 Oct 3. pii: S0002-9440(13)00614-7. doi: 10.1016/j.ajpath.2013.08.026.

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$ 350.00
Cat# AT1749a
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Availability: 7-10 days
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