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DNAJA2 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant DNAJA2.

     
  • IF - DNAJA2 Antibody (monoclonal) (M01) AT1787a
    Immunofluorescence of monoclonal antibody to DNAJA2 on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - DNAJA2 Antibody (monoclonal) (M01) AT1787a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (71.06 KDa) .
    detail
  • WB - DNAJA2 Antibody (monoclonal) (M01) AT1787a
    DNAJA2 monoclonal antibody (M01), clone 2A11-F2 Western Blot analysis of DNAJA2 expression in A-431 ( (Cat # AT1787a )
    detail
  • E - DNAJA2 Antibody (monoclonal) (M01) AT1787a
    Detection limit for recombinant GST tagged DNAJA2 is approximately 3ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession O60884
Other Accession BC013044
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 2A11-F2
Calculated MW 45746 Da
Additional Information
Gene ID 10294
Other Names DnaJ homolog subfamily A member 2, Cell cycle progression restoration gene 3 protein, Dnj3, Dj3, HIRA-interacting protein 4, Renal carcinoma antigen NY-REN-14, DNAJA2, CPR3, HIRIP4
Target/Specificity DNAJA2 (AAH13044, 1 a.a. ~ 412 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsDNAJA2 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain. The product of this gene works as a cochaperone of Hsp70s in protein folding and mitochondrial protein import in vitro.

References

1.Cell line specific modulation of extracellular a?](42) by hsp40.Carnini A, Scott LO, Ahrendt E, Proft J, Winkfein RJ, Kim SW, Colicos MA, Braun JE.PLoS One. 2012;7(5):e37755. Epub 2012 May 29.2.Quercetin targets cysteine string protein (CSPalpha) and impairs synaptic transmission.Xu F, Proft J, Gibbs S, Winkfein B, Johnson JN, Syed N, Braun JE.PLoS One. 2010 Jun 10;5(6):e11045.3.RDJ2 (DNAJA2) chaperones neural G protein signaling pathways.Rosales-Hernandez A, Beck KE, Zhao X, Braun AP, Braun JE.Cell Stress Chaperones. 2009 Jan;14(1):71-82. Epub 2008 Jul 2.

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$ 350.00
Cat# AT1787a
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