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DPH1 Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a partial recombinant DPH1.

  • WB - DPH1 Antibody (monoclonal) (M02) AT1812a
    DPH1 monoclonal antibody (M02), clone 2C5 Western Blot analysis of DPH1 expression in HeLa ( Cat # L013V1 ).
  • IF - DPH1 Antibody (monoclonal) (M02) AT1812a
    Immunofluorescence of monoclonal antibody to DPH1 on HeLa cell. [antibody concentration 10 ug/ml]
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9BZG8
Other Accession NM_001383
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names 2C5
Calculated MW 48805 Da
Additional Information
Gene ID 1801
Other Names Diphthamide biosynthesis protein 1, DPH1 homolog, HsDph1, Diphthamide biosynthesis protein 2 homolog-like 1, DPH-like 1, DPH2-like 1, Diphthamide biosynthesis protein 2-like, Ovarian cancer-associated gene 1 protein, DPH1, DPH2L, DPH2L1, OVCA1
Target/Specificity DPH1 (NP_001374, 216 a.a. ~ 324 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsDPH1 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).


Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2. Liu S, et al. Mol Cell Biol, 2004 Nov. PMID 15485916.Complete sequencing and characterization of 21,243 full-length human cDNAs. Ota T, et al. Nat Genet, 2004 Jan. PMID 14702039.Refinement of a 400-kb critical region allows genotypic differentiation between isolated lissencephaly, Miller-Dieker syndrome, and other phenotypes secondary to deletions of 17p13.3. Cardoso C, et al. Am J Hum Genet, 2003 Apr. PMID 12621583.Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Strausberg RL, et al. Proc Natl Acad Sci U S A, 2002 Dec 24. PMID 12477932.Cloning, structure, and expression of the mouse Ovca1 gene. Chen CM, et al. Biochem Biophys Res Commun, 2001 Sep 7. PMID 11527402.

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$ 315.00
Cat# AT1812a
(40 western blots)
Availability: 7-10 days
Bulk Size
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