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>   home   >   Products   >   Primary Antibodies   >   EAP30 Antibody (monoclonal) (M01)   

EAP30 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant SNF8.

  • WB - EAP30 Antibody (monoclonal) (M01) AT1841a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
  • WB - EAP30 Antibody (monoclonal) (M01) AT1841a
    Western Blot analysis of SNF8 expression in transfected 293T cell line by SNF8 monoclonal antibody (M01), clone 6B11.

    Lane 1: SNF8 transfected lysate (Predicted MW: 28.9 KDa).
    Lane 2: Non-transfected lysate.
  • E - EAP30 Antibody (monoclonal) (M01) AT1841a
    Detection limit for recombinant GST tagged SNF8 is approximately 1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q96H20
Other Accession NM_007241
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 6B11
Calculated MW 28864 Da
Additional Information
Gene ID 11267
Other Names Vacuolar-sorting protein SNF8, ELL-associated protein of 30 kDa, ESCRT-II complex subunit VPS22, hVps22, SNF8, EAP30
Target/Specificity SNF8 (NP_009172, 23 a.a. ~ 122 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsEAP30 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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SNF8, VPS25 (MIM 610907), and VPS36 (MIM 610903) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. SNF8, VPS25, and VPS36 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).


Vps22/EAP30 in ESCRT-II mediates endosomal sorting of growth factor and chemokine receptors destined for lysosomal degradation. Maler?d L, et al. Traffic, 2007 Nov. PMID 17714434.Human ESCRT-II complex and its role in human immunodeficiency virus type 1 release. Langelier C, et al. J Virol, 2006 Oct. PMID 16973552.Degradation of endocytosed epidermal growth factor and virally ubiquitinated major histocompatibility complex class I is independent of mammalian ESCRTII. Bowers K, et al. J Biol Chem, 2006 Feb 24. PMID 16371348.Towards a proteome-scale map of the human protein-protein interaction network. Rual JF, et al. Nature, 2005 Oct 20. PMID 16189514.Eap45 in mammalian ESCRT-II binds ubiquitin via a phosphoinositide-interacting GLUE domain. Slagsvold T, et al. J Biol Chem, 2005 May 20. PMID 15755741.

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$ 315.00
Cat# AT1841a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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