EAP30 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant SNF8.
|Application ||WB, E|
|Calculated MW||28864 Da|
|Other Names||Vacuolar-sorting protein SNF8, ELL-associated protein of 30 kDa, ESCRT-II complex subunit VPS22, hVps22, SNF8, EAP30|
|Target/Specificity||SNF8 (NP_009172, 23 a.a. ~ 122 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Format||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.|
|Precautions||EAP30 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
SNF8, VPS25 (MIM 610907), and VPS36 (MIM 610903) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. SNF8, VPS25, and VPS36 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).
Vps22/EAP30 in ESCRT-II mediates endosomal sorting of growth factor and chemokine receptors destined for lysosomal degradation. Maler?d L, et al. Traffic, 2007 Nov. PMID 17714434.Human ESCRT-II complex and its role in human immunodeficiency virus type 1 release. Langelier C, et al. J Virol, 2006 Oct. PMID 16973552.Degradation of endocytosed epidermal growth factor and virally ubiquitinated major histocompatibility complex class I is independent of mammalian ESCRTII. Bowers K, et al. J Biol Chem, 2006 Feb 24. PMID 16371348.Towards a proteome-scale map of the human protein-protein interaction network. Rual JF, et al. Nature, 2005 Oct 20. PMID 16189514.Eap45 in mammalian ESCRT-II binds ubiquitin via a phosphoinositide-interacting GLUE domain. Slagsvold T, et al. J Biol Chem, 2005 May 20. PMID 15755741.
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