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>   home   >   Products   >   Primary Antibodies   >   EIF2S1 Antibody (monoclonal) (M01)   

EIF2S1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant EIF2S1.

  • WB - EIF2S1 Antibody (monoclonal) (M01) AT1875a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (60.39 KDa) .
  • WB - EIF2S1 Antibody (monoclonal) (M01) AT1875a
    EIF2S1 monoclonal antibody (M01), clone 3H12-C11 Western Blot analysis of EIF2S1 expression in HeLa ( (Cat # AT1875a )
  • WB - EIF2S1 Antibody (monoclonal) (M01) AT1875a
    EIF2S1 monoclonal antibody (M01), clone 3H12-C11. Western Blot analysis of EIF2S1 expression in Hela S3 NE ( (Cat # AT1875a )
  • WB - EIF2S1 Antibody (monoclonal) (M01) AT1875a
    Western Blot analysis of EIF2S1 expression in transfected 293T cell line by EIF2S1 monoclonal antibody (M01), clone 3H12-C11.

    Lane 1: EIF2S1 transfected lysate(36.1 KDa).
    Lane 2: Non-transfected lysate.
  • IF - EIF2S1 Antibody (monoclonal) (M01) AT1875a
    Immunofluorescence of monoclonal antibody to EIF2S1 on HeLa cell. [antibody concentration 10 ug/ml]
  • E - EIF2S1 Antibody (monoclonal) (M01) AT1875a
    Detection limit for recombinant GST tagged EIF2S1 is approximately 0.3ng/ml as a capture antibody.
  • WB - EIF2S1 Antibody (monoclonal) (M01) AT1875a
    Western blot analysis of EIF2S1 over-expressed 293 cell line, cotransfected with EIF2S1 Validated Chimera RNAi ( (Cat # AT1875a )
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P05198
Other Accession BC002513
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 3H12-C11
Calculated MW 36112 Da
Additional Information
Gene ID 1965
Other Names Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, EIF2S1, EIF2A
Target/Specificity EIF2S1 (AAH02513.1, 1 a.a. ~ 315 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsEIF2S1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).


1.IGF2BP1 enhances HCV IRES-mediated translation initiation via the 3'UTR.Weinlich S, Huttelmaier S, Schierhorn A, Behrens SE, Ostareck-Lederer A, Ostareck DH.RNA. 2009 Aug;15(8):1528-42. Epub 2009 Jun 18.

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$ 315.00
Cat# AT1875a
(40 western blots)
Availability: 7-10 days
Bulk Size
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