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ERF Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a partial recombinant ERF.

     
  • IF - ERF Antibody (monoclonal) (M02) AT1940a
    Immunofluorescence of monoclonal antibody to ERF on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - ERF Antibody (monoclonal) (M02) AT1940a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.73 KDa) .
    detail
  • WB - ERF Antibody (monoclonal) (M02) AT1940a
    ERF monoclonal antibody (M02), clone 3F11. Western Blot analysis of ERF expression in Raw 264.7.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF
Primary Accession P50548
Other Accession BC022231
Reactivity Human, Mouse
Host mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names 3F11
Calculated MW 58703 Da
Additional Information
Gene ID 2077
Other Names ETS domain-containing transcription factor ERF, Ets2 repressor factor, PE-2, ERF
Target/Specificity ERF (AAH22231, 181 a.a. ~ 290 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsERF Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)
citation

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Background

Members of the ETS family of transcription factors, such as ERF, regulate cell proliferation and differentiation. They share a highly conserved DNA-binding domain, the ETS domain, that recognizes the sequence GGAA/T (de Castro et al., 1997 [PubMed 9192842]). For further information on ETS transcription factors, see ETS1 (MIM 164720).

References

The RAS-dependent ERF control of cell proliferation and differentiation is mediated by c-Myc repression. Verykokakis M, et al. J Biol Chem, 2007 Oct 12. PMID 17699159.Differential repression of c-myc and cdc2 gene expression by ERF and PE-1/METS. Hester KD, et al. Cell Cycle, 2007 Jul 1. PMID 17525531.Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Olsen JV, et al. Cell, 2006 Nov 3. PMID 17081983.A probability-based approach for high-throughput protein phosphorylation analysis and site localization. Beausoleil SA, et al. Nat Biotechnol, 2006 Oct. PMID 16964243.The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.

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$ 350.00
Cat# AT1940a
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