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ESM1 Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a partial recombinant ESM1.

     
  • WB - ESM1 Antibody (monoclonal) (M02) AT1949a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
    detail
  • WB - ESM1 Antibody (monoclonal) (M02) AT1949a
    Western Blot analysis of ESM1 expression in transfected 293T cell line by ESM1 monoclonal antibody (M02), clone 6D4.

    Lane 1: ESM1 transfected lysate(20.1 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • IHC - ESM1 Antibody (monoclonal) (M02) AT1949a
    Immunoperoxidase of monoclonal antibody to ESM1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]
    detail
  • E - ESM1 Antibody (monoclonal) (M02) AT1949a
    Detection limit for recombinant GST tagged ESM1 is approximately 0.03ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession Q9NQ30
Other Accession NM_007036
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names 6D4
Calculated MW 20095 Da
Additional Information
Gene ID 11082
Other Names Endothelial cell-specific molecule 1, ESM-1, ESM1
Target/Specificity ESM1 (NP_008967, 85 a.a. ~ 184 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsESM1 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. Two transcript variants encoding different isoforms have been found for this gene.

References

1.ESM-1 silencing decreased cell survival, migration, and invasion and modulated cell cycle progression in hepatocellular carcinoma.Kang YH, Ji NY, Lee CI, Lee HG, Kim JW, Yeom YI, Kim DG, Yoon SK, Kim JW, Park PJ, Song EY.Amino Acids. 2010 Sep 7. [Epub ahead of print]2.Identification of endothelial cell-specific molecule-1 as a potential serum marker for colorectal cancer.Ji NY, Kim YH, Jang YJ, Kang YH, Lee CI, Kim JW, Yeom YI, Chun HK, Choi YH, Kim JH, Kim JW, Lee HG, Song EY.Cancer Sci. 2010 Jul 30. [Epub ahead of print]3.Overexpression of Endothelial Cell Specific Molecule-1 (ESM-1) in Gastric Cancer.Liu N, Zhang LH, Du H, Hu Y, Zhang GG, Wang XH, Li JY, Ji JF.Ann Surg Oncol. 2010 Apr 10. [Epub ahead of print]

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$ 350.00
Cat# AT1949a
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Availability: 7-10 days
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