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F8 Antibody (monoclonal) (M03)

Mouse monoclonal antibody raised against a partial recombinant F8.

  • E - F8 Antibody (monoclonal) (M03) AT1980a
    Detection limit for recombinant GST tagged F8 is approximately 3ng/ml as a capture antibody.
  •  - F8 Antibody (monoclonal) (M03) AT1980a
    Proximity Ligation Analysis of protein-protein interactions between CALR and F8. HeLa cells were stained with anti-CALR rabbit purified polyclonal 1:1200 and anti-F8 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P00451
Other Accession NM_000132
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names 1E9
Calculated MW 267009 Da
Additional Information
Gene ID 2157
Other Names Coagulation factor VIII, Antihemophilic factor, AHF, Procoagulant component, Factor VIIIa heavy chain, 200 kDa isoform, Factor VIIIa heavy chain, 92 kDa isoform, Factor VIII B chain, Factor VIIIa light chain, F8, F8C
Target/Specificity F8 (NP_000123, 213 a.a. ~ 312 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsF8 Antibody (monoclonal) (M03) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder.


Variation at the NFATC2 Locus Increases the Risk of Thiazolinedinedione-Induced Edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) Study. Bailey SD, et al. Diabetes Care, 2010 Jul 13. PMID 20628086.Factor XIII A subunit Val34Leu polymorphism in patients suffering atherothrombotic ischemic stroke. Shemirani AH, et al. Thromb Res, 2010 Aug. PMID 20609463.Synergism between factor XII -4C>T and factor XIII Val34Leu polymorphisms in fibrinolytic therapy in acute myocardial infarction. Hern?ndez-Romero D, et al. Thromb Haemost, 2010 Sep. PMID 20589311.Activation of human endothelial cells from specific vascular beds induces the release of a FVIII storage pool. Shahani T, et al. Blood, 2010 Jun 10. PMID 20351306.New genetic associations detected in a host response study to hepatitis B vaccine. Davila S, et al. Genes Immun, 2010 Apr. PMID 20237496.

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$ 315.00
Cat# AT1980a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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