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FGF12 Antibody (monoclonal) (M10)

Mouse monoclonal antibody raised against a full length recombinant FGF12.

     
  • WB - FGF12 Antibody (monoclonal) (M10) AT2032a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (45.65 KDa) .
    detail
  • WB - FGF12 Antibody (monoclonal) (M10) AT2032a
    Western Blot analysis of FGF12 expression in transfected 293T cell line by FGF12 monoclonal antibody (M10), clone 1D9.

    Lane 1: FGF12 transfected lysate(27.4 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • IHC - FGF12 Antibody (monoclonal) (M10) AT2032a
    Immunoperoxidase of monoclonal antibody to FGF12 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 3 ug/ml]
    detail
  • E - FGF12 Antibody (monoclonal) (M10) AT2032a
    Detection limit for recombinant GST tagged FGF12 is approximately 0.1ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession P61328
Other Accession BC022524
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 1D9
Calculated MW 27399 Da
Additional Information
Gene ID 2257
Other Names Fibroblast growth factor 12, FGF-12, Fibroblast growth factor homologous factor 1, FHF-1, Myocyte-activating factor, FGF12, FGF12B, FHF1
Target/Specificity FGF12 (AAH22524, 1 a.a. ~ 181 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsFGF12 Antibody (monoclonal) (M10) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This growth factor lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted. The specific function of this gene has not yet been determined. Two alternatively spliced transcript variants encoding distinct isoforms have been reported.

References

1.Genome-wide analysis of chromosomal alterations in patients with esophageal squamous cell carcinoma exposed to tobacco and betel quid from high-risk area in India.Chattopadhyay I, Singh A, Phukan R, Purkayastha J, Kataki A, Mahanta J, Saxena S, Kapur S.Mutat Res. 2010 Jan 18. [Epub ahead of print]

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$ 350.00
Cat# AT2032a
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