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GAPDH Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant GAPDH.

     
  • WB - GAPDH Antibody (monoclonal) (M01) AT2151a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.84 KDa) .
    detail
  • WB - GAPDH Antibody (monoclonal) (M01) AT2151a
    GAPDH monoclonal antibody (M01), clone 3C2. Western Blot analysis of GAPDH expression in HeLa ( (Cat # AT2151a )
    detail
  • WB - GAPDH Antibody (monoclonal) (M01) AT2151a
    GAPDH monoclonal antibody (M01), clone 3C2. Western Blot analysis of GAPDH expression in PC-12((Cat # AT2151a )
    detail
  • WB - GAPDH Antibody (monoclonal) (M01) AT2151a
    GAPDH monoclonal antibody (M01), clone 3C2. Western Blot analysis of GAPDH expression in Raw 264.7((Cat # AT2151a )
    detail
  • WB - GAPDH Antibody (monoclonal) (M01) AT2151a
    GAPDH monoclonal antibody (M01), clone 3C2 Western Blot analysis of GAPDH expression in A-431 ( (Cat # AT2151a )
    detail
  • WB - GAPDH Antibody (monoclonal) (M01) AT2151a
    GAPDH monoclonal antibody (M01), clone 3C2. Western Blot analysis of GAPDH expression in NIH/3T3((Cat # AT2151a )
    detail
  • WB - GAPDH Antibody (monoclonal) (M01) AT2151a
    Western Blot analysis of GAPDH expression in transfected 293T cell line by GAPDH monoclonal antibody (M01), clone 3C2.

    Lane 1: GAPDH transfected lysate(36.1 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - GAPDH Antibody (monoclonal) (M01) AT2151a
    Detection limit for recombinant GST tagged GAPDH is 0.03 ng/ml as a capture antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P04406
Other Accession NM_002046
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 3C2
Calculated MW 36053 Da
Additional Information
Gene ID 2597
Other Names Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, 2699-, GAPDH, GAPD
Target/Specificity GAPDH (NP_002037, 226 a.a. ~ 335 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsGAPDH Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.

References

1.Receptor for Activated Protein Kinase C: Requirement for Efficient MicroRNA Function and Reduced Expression in Hepatocellular Carcinoma.Otsuka M, Takata A, Yoshikawa T, Kojima K, Kishikawa T, Shibata C, Takekawa M, Yoshida H, Omata M, Koike K.PLoS One. 2011;6(9):e24359. Epub 2011 Sep 15.2.Expression and roles of Slit/Robo in human ovarian cancer.Dai CF, Jiang YZ, Li Y, Wang K, Liu PS, Patankar MS, Zheng J.Histochem Cell Biol. 2011 Apr 5. [Epub ahead of print]3.Anti-angiogenic effect of triterpenoidal saponins from Polygala senega.Arai M, Hayashi A, Sobou M, Ishida S, Kawachi T, Kotoku N, Kobayashi M.J Nat Med. 2010 Nov 2. [Epub ahead of print]4.The Effect of Allelic Variation in Aldo-Keto Reductase 1C2 on the In Vitro Metabolism of Dihydrotestosterone.Takahashi RH, Grigliatti TA, Reid RE, Riggs KW.J Pharmacol Exp Ther. 2009 Jun;329(3):1032-9. Epub 2009 Mar 3.

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$ 350.00
Cat# AT2151a
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Availability: 7-10 days
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