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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   GAPDH Antibody (monoclonal) (M03)   

GAPDH Antibody (monoclonal) (M03)

Mouse monoclonal antibody raised against a partial recombinant GAPDH.

  • WB - GAPDH Antibody (monoclonal) (M03) AT2152a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.84 KDa) .
  • WB - GAPDH Antibody (monoclonal) (M03) AT2152a
    GAPDH monoclonal antibody (M03), clone 1G5. Western Blot analysis of GAPDH expression in HeLa ( (Cat # AT2152a )
  • IP - GAPDH Antibody (monoclonal) (M03) AT2152a
    Immunoprecipitation of GAPDH transfected lysate using anti-GAPDH monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with GAPDH MaxPab rabbit polyclonal antibody.
  • E - GAPDH Antibody (monoclonal) (M03) AT2152a
    Detection limit for recombinant GST tagged GAPDH is approximately 1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P04406
Other Accession NM_002046
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 1G5
Calculated MW 36053 Da
Additional Information
Gene ID 2597
Other Names Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, 2699-, GAPDH, GAPD
Target/Specificity GAPDH (NP_002037, 226 a.a. ~ 335 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsGAPDH Antibody (monoclonal) (M03) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.


Inhibition of glyceraldehyde-3-phosphate dehydrogenase activity by antibodies present in the cerebrospinal fluid of patients with multiple sclerosis. K?lln J, et al. J Immunol, 2010 Aug 1. PMID 20610654.Proteome analysis of the thalamus and cerebrospinal fluid reveals glycolysis dysfunction and potential biomarkers candidates for schizophrenia. Martins-de-Souza D, et al. J Psychiatr Res, 2010 May 14. PMID 20471030.Sex-specific proteome differences in the anterior cingulate cortex of schizophrenia. Martins-de-Souza D, et al. J Psychiatr Res, 2010 Apr 8. PMID 20381070.Identification of melanoma antigens using a Serological Proteome Approach (SERPA). Suzuki A, et al. Cancer Genomics Proteomics, 2010 Jan-Feb. PMID 20181627.siah-1 Protein is necessary for high glucose-induced glyceraldehyde-3-phosphate dehydrogenase nuclear accumulation and cell death in Muller cells. Yego EC, et al. J Biol Chem, 2010 Jan 29. PMID 19940145.

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$ 315.00
Cat# AT2152a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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