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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   GCA Antibody (monoclonal) (M01)   

GCA Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full-length recombinant GCA.

  • WB - GCA Antibody (monoclonal) (M01) AT2171a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (49.61 KDa) .
  • WB - GCA Antibody (monoclonal) (M01) AT2171a
    GCA monoclonal antibody (M01), clone 2F5 Western Blot analysis of GCA expression in MCF-7 ( (Cat # AT2171a )
  • IF - GCA Antibody (monoclonal) (M01) AT2171a
    Immunofluorescence of monoclonal antibody to GCA on MCF-7 cell . [antibody concentration 10 ug/ml]
  • E - GCA Antibody (monoclonal) (M01) AT2171a
    Detection limit for recombinant GST tagged GCA is 0.03 ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P28676
Other Accession BC005214
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 2F5
Calculated MW 24010 Da
Additional Information
Gene ID 25801
Other Names Grancalcin, GCA, GCL
Target/Specificity GCA (AAH05214, 1 a.a. ~ 217 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsGCA Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation.


New genetic associations detected in a host response study to hepatitis B vaccine. Davila S, et al. Genes Immun, 2010 Apr. PMID 20237496.IFIH1-GCA-KCNH7 locus is not associated with genetic susceptibility to multiple sclerosis in French patients. Couturier N, et al. Eur J Hum Genet, 2009 Jun. PMID 19156166.IFIH1-GCA-KCNH7 locus: influence on multiple sclerosis risk. Mart?nez A, et al. Eur J Hum Genet, 2008 Jul. PMID 18285833.The association between the IFIH1 locus and type 1 diabetes. Qu HQ, et al. Diabetologia, 2008 Mar. PMID 18071670.A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. Lim J, et al. Cell, 2006 May 19. PMID 16713569.

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$ 315.00
Cat# AT2171a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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