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GHRL Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant GHRL.

     
  • WB - GHRL Antibody (monoclonal) (M01) AT2199a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (38.61 KDa) .
    detail
  • WB - GHRL Antibody (monoclonal) (M01) AT2199a
    GHRL monoclonal antibody (M01), clone 2F4. Western Blot analysis of GHRL expression in HeLa.
    detail
  • IHC - GHRL Antibody (monoclonal) (M01) AT2199a
    Immunoperoxidase of monoclonal antibody to GHRL on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.5 ug/ml]
    detail
  • E - GHRL Antibody (monoclonal) (M01) AT2199a
    Detection limit for recombinant GST tagged GHRL is approximately 0.1ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession Q9UBU3
Other Accession BC025791
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 2F4
Calculated MW 12911 Da
Additional Information
Gene ID 51738
Other Names Appetite-regulating hormone, Growth hormone secretagogue, Growth hormone-releasing peptide, Motilin-related peptide, Protein M46, Ghrelin-27, Ghrelin-28, Ghrelin, Obestatin, GHRL, MTLRP
Target/Specificity GHRL (AAH25791.1, 1 a.a. ~ 117 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsGHRL Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
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Background

This gene encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor and is involved in regulating growth hormone release. Obestatin was initially reported to be an endogenous ligand for the orphan G protein-coupled receptor GPR39 and was involved in satiety and decreased food intake; however, these findings are controversial. Recent reports show that obestatin is involved in inhibiting thirst and anxiety, improving memory, regulating sleep, affecting cell proliferation, and increasing the secretion of pancreatic juice enzymes. Alternative promoters and alternative splicing result in multiple transcript variants, some of which encode different protein isoforms and some of which do not encode a protein but may regulate the ghrelin-obestatin preproprotein expression. In addition, antisense transcripts for this gene have been identified and may also function in regulation of the ghrelin-obestatin preproprotein expression.

References

1.Mapping of Ghrelin Gene Expression and Cell Distribution in the Stomach of Morbidly Obese Patients-a Possible Guide for Efficient Sleeve Gastrectomy Construction.Goitein D, Lederfein D, Tzioni R, Berkenstadt H, Venturero M, Rubin M.Obes Surg. 2012 Jan 10. [Epub ahead of print]

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$ 350.00
Cat# AT2199a
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Availability: 7-10 days
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