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GJB3 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant GJB3.

     
  • IF - GJB3 Antibody (monoclonal) (M01) AT2202a
    Immunofluorescence of monoclonal antibody to GJB3 on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - GJB3 Antibody (monoclonal) (M01) AT2202a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (55.44 KDa) .
    detail
  • WB - GJB3 Antibody (monoclonal) (M01) AT2202a
    GJB3 monoclonal antibody (M01), clone 3B4-1B3 Western Blot analysis of GJB3 expression in HeLa ( (Cat # AT2202a )
    detail
  • WB - GJB3 Antibody (monoclonal) (M01) AT2202a
    Western Blot analysis of GJB3 expression in transfected 293T cell line by GJB3 monoclonal antibody (M01), clone 3B4-1B3.

    Lane 1: GJB3 transfected lysate(31 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - GJB3 Antibody (monoclonal) (M01) AT2202a
    Detection limit for recombinant GST tagged GJB3 is approximately 0.3ng/ml as a capture antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession O75712
Other Accession BC012918
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 3B4-1B3
Calculated MW 30818 Da
Additional Information
Gene ID 2707
Other Names Gap junction beta-3 protein, Connexin-31, Cx31, GJB3, CX31
Target/Specificity GJB3 (AAH12918.1, 1 a.a. ~ 270 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsGJB3 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene can cause non-syndromic deafness or erythrokeratodermia variabilis, a skin disorder. Alternative splicing results in multiple transcript variants encoding the same protein.

References

1.EKV mutant connexin 31 associated cell death is mediated by ER stress.Tattersall D, Scott CA, Gray C, Zicha D, Kelsell DP.Hum Mol Genet. 2009 Dec 15;18(24):4734-45. Epub 2009 Sep 14.

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$ 350.00
Cat# AT2202a
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