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GLS Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant GLS.

  • WB - GLS Antibody (monoclonal) (M01) AT2211a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (35.64 KDa) .
  • IF - GLS Antibody (monoclonal) (M01) AT2211a
    Immunofluorescence of monoclonal antibody to GLS on HeLa cell. [antibody concentration 10 ug/ml]
  • E - GLS Antibody (monoclonal) (M01) AT2211a
    Detection limit for recombinant GST tagged GLS is approximately 0.3ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O94925
Other Accession NM_014905
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 5C4
Calculated MW 73461 Da
Additional Information
Gene ID 2744
Other Names Glutaminase kidney isoform, mitochondrial, GLS, K-glutaminase, L-glutamine amidohydrolase, GLS, GLS1, KIAA0838
Target/Specificity GLS (NP_055720, 580 a.a. ~ 669 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsGLS Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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Sahai (1983) [PubMed 6825316] demonstrated phosphate-activated glutaminase (EC in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter (Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.


1.Combination of neonatal PolyI:C and adolescent phencyclidine treatments is required to induce behavioral abnormalities with overexpression of GLAST in adult mice.Hida H, Mouri A, Ando Y, Mori K, Mamiya T, Iwamoto K, Ozaki N, Yamada K, Nabeshima T, Noda YBehav Brain Res. 2013 Sep 20. pii: S0166-4328(13)00576-7. doi: 10.1016/j.bbr.2013.09.026.2.Control of glutamine metabolism by the tumor suppressor Rb.Reynolds MR, Lane AN, Robertson B, Kemp S, Liu Y, Hill BG, Dean DC, Clem BFOncogene. 2013 Jan 28. doi: 10.1038/onc.2012.635.3.Mitochondrial localization and structure-based phosphate activation mechanism of Glutaminase C with implications for cancer metabolism.Cassago A, Ferreira AP, Ferreira IM, Fornezari C, Gomes ER, Greene KS, Pereira HM, Garratt RC, Dias SM, Ambrosio AL.Proc Natl Acad Sci U S A. 2012 Jan 24;109(4):1092-7. Epub 2012 Jan 6.4.Premature senescence of human endothelial cells induced by inhibition of glutaminase.Unterluggauer H, Mazurek S, Lener B, Hutter E, Eigenbrodt E, Zwerschke W, Jansen-Durr P.Biogerontology. 2008 Aug;9(4):247-59. Epub 2008 Mar 4.

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$ 315.00
Cat# AT2211a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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