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GOLM1 Antibody (monoclonal) (M06)

Mouse monoclonal antibody raised against a partial recombinant GOLM1.

     
  • IF - GOLM1 Antibody (monoclonal) (M06) AT2239a
    Immunofluorescence of monoclonal antibody to GOLM1 on HeLa cell . [antibody concentration 10 ug/ml]
    detail
  • WB - GOLM1 Antibody (monoclonal) (M06) AT2239a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
    detail
  • WB - GOLM1 Antibody (monoclonal) (M06) AT2239a
    Western Blot analysis of GOLM1 expression in transfected 293T cell line by GOLM1 monoclonal antibody (M06), clone 5B10.

    Lane 1: GOLM1 transfected lysate(45.3 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • IHC - GOLM1 Antibody (monoclonal) (M06) AT2239a
    Immunoperoxidase of monoclonal antibody to GOLM1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3 ug/ml]
    detail
  • E - GOLM1 Antibody (monoclonal) (M06) AT2239a
    Detection limit for recombinant GST tagged GOLM1 is approximately 0.1ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS: 1
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, E
Primary Accession Q8NBJ4
Other Accession NM_016548
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 5B10
Calculated MW 45333 Da
Additional Information
Gene ID 51280
Other Names Golgi membrane protein 1, Golgi membrane protein GP73, Golgi phosphoprotein 2, GOLM1, C9orf155, GOLPH2
Target/Specificity GOLM1 (NP_057632, 302 a.a. ~ 401 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsGOLM1 Antibody (monoclonal) (M06) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations ( 0 )

Background

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes proteins synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this gene has been observed to be upregulated in response to viral infection. Alternatively spliced transcript variants encoding the same protein have been described for this gene.

References

1.The HOPE fixation technique - a promising alternative to common prostate cancer biobanking approaches.Braun M, Menon R, Nikolov P, Kirsten R, Petersen K, Schilling D, Schott C, Gundisch S, Fend F, Becker KF, Perner S.BMC Cancer. 2011 Dec 7;11:511.2.ERG rearrangement in small cell prostatic and lung cancer.Scheble VJ, Braun M, Wilbertz T, Stiedl AC, Petersen K, Schilling D, Reischl M, Seitz G, Fend F, Kristiansen G, Perner S.Histopathology. 2010 Jun;56(7):937-43.3.GOLPH2 protein expression as a novel tissue biomarker for prostate cancer: implications for tissue-based diagnostics.Kristiansen G, Fritzsche FR, Wassermann K, Jager C, Tolls A, Lein M, Stephan C, Jung K, Pilarsky C, Dietel M, Moch H.Br J Cancer. 2008 Sep 16;99(6):939-48.4.GOLPH2 and MYO6: putative prostate cancer markers localized to the Golgi apparatus.Wei S, Dunn TA, Isaacs WB, De Marzo AM, Luo J.Prostate. 2008 Sep 15;68(13):1387-95.

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$ 350.00
Cat# AT2239a
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Availability: 7-10 days
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