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GOLPH4 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant GOLPH4.

     
  • IF - GOLPH4 Antibody (monoclonal) (M01) AT2240a
    Immunofluorescence of monoclonal antibody to GOLPH4 on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - GOLPH4 Antibody (monoclonal) (M01) AT2240a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.3 KDa) .
    detail
  • WB - GOLPH4 Antibody (monoclonal) (M01) AT2240a
    GOLPH4 monoclonal antibody (M01), clone 5E11 Western Blot analysis of GOLPH4 expression in HeLa ( (Cat # AT2240a )
    detail
  • E - GOLPH4 Antibody (monoclonal) (M01) AT2240a
    Detection limit for recombinant GST tagged GOLPH4 is approximately 0.03ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession O00461
Other Accession NM_014498
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 5E12
Calculated MW 81880 Da
Additional Information
Gene ID 27333
Other Names Golgi integral membrane protein 4, Golgi integral membrane protein, cis, GIMPc, Golgi phosphoprotein 4, Golgi-localized phosphoprotein of 130 kDa, Golgi phosphoprotein of 130 kDa, GOLIM4, GIMPC, GOLPH4, GPP130
Target/Specificity GOLPH4 (NP_055311.1, 473 a.a. ~ 568 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsGOLPH4 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi-resident protein. It may process proteins synthesized in the rough endoplasmic reticulum and assist in the transport of protein cargo through the Golgi apparatus.

References

Manganese-induced trafficking and turnover of the cis-Golgi glycoprotein GPP130. Mukhopadhyay S, et al. Mol Biol Cell, 2010 Apr 1. PMID 20130081.Many sequence variants affecting diversity of adult human height. Gudbjartsson DF, et al. Nat Genet, 2008 May. PMID 18391951.Toward a confocal subcellular atlas of the human proteome. Barbe L, et al. Mol Cell Proteomics, 2008 Mar. PMID 18029348.Both post-Golgi and intra-Golgi cycling affect the distribution of the Golgi phosphoprotein GPP130. Starr T, et al. Traffic, 2007 Sep. PMID 17605763.Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Kimura K, et al. Genome Res, 2006 Jan. PMID 16344560.

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$ 350.00
Cat# AT2240a
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Availability: 7-10 days
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