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GSN Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant GSN.

     
  • IP - GSN Antibody (monoclonal) (M01) AT2272a
    Immunoprecipitation of GSN transfected lysate using anti-GSN monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with GSN MaxPab rabbit polyclonal antibody.
    detail
  • WB - GSN Antibody (monoclonal) (M01) AT2272a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.84 KDa) .
    detail
  • WB - GSN Antibody (monoclonal) (M01) AT2272a
    GSN monoclonal antibody (M01), clone 3G5 Western Blot analysis of GSN expression in HeLa ( (Cat # AT2272a )
    detail
  • WB - GSN Antibody (monoclonal) (M01) AT2272a
    Western Blot analysis of GSN expression in transfected 293T cell line by GSN monoclonal antibody (M01), clone 3G5.

    Lane 1: GSN transfected lysate(85.7 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • IHC - GSN Antibody (monoclonal) (M01) AT2272a
    Immunoperoxidase of monoclonal antibody to GSN on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 1 ug/ml]
    detail
  • E - GSN Antibody (monoclonal) (M01) AT2272a
    Detection limit for recombinant GST tagged GSN is approximately 0.03ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IP, E
Primary Accession P06396
Other Accession BC026033
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 3G5
Calculated MW 85698 Da
Additional Information
Gene ID 2934
Other Names Gelsolin, AGEL, Actin-depolymerizing factor, ADF, Brevin, GSN
Target/Specificity GSN (AAH26033, 673 a.a. ~ 782 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsGSN Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene binds to the plus ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene.

References

Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.The potential role of plasma gelsolin in dialysis-related protein-energy wasting. Lee PS, et al. Blood Purif, 2010. PMID 20093811.Gelsolin in human colon adenocarcinoma cells with different metastatic potential. Litwin M, et al. Acta Biochim Pol, 2009. PMID 19956804.The 8 and 5 kDa fragments of plasma gelsolin form amyloid fibrils by a nucleated polymerization mechanism, while the 68 kDa fragment is not amyloidogenic. Solomon JP, et al. Biochemistry, 2009 Dec 8. PMID 19904968.Proteomics of tumor-specific proteins in cerebrospinal fluid of patients with astrocytoma: usefulness of gelsolin protein. Ohnishi M, et al. Pathol Int, 2009 Nov. PMID 19883430.

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$ 350.00
Cat# AT2272a
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Availability: 7-10 days
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