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HAL Antibody (monoclonal) (M04)

Mouse monoclonal antibody raised against a partial recombinant HAL.

     
  • IP - HAL Antibody (monoclonal) (M04) AT2311a
    Immunoprecipitation of HAL transfected lysate using anti-HAL monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HAL MaxPab rabbit polyclonal antibody.
    detail
  • WB - HAL Antibody (monoclonal) (M04) AT2311a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.11 KDa) .
    detail
  • WB - HAL Antibody (monoclonal) (M04) AT2311a
    Western Blot analysis of HAL expression in transfected 293T cell line by HAL monoclonal antibody (M04), clone 4F2.

    Lane 1: HAL transfected lysate(72.698 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IP
Primary Accession P42357
Other Accession NM_002108
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 4F2
Calculated MW 72698 Da
Additional Information
Gene ID 3034
Other Names Histidine ammonia-lyase, Histidase, HAL, HIS
Target/Specificity HAL (NP_002099, 558 a.a. ~ 658 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsHAL Antibody (monoclonal) (M04) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

Histidine ammonia-lyase is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid. Histidine ammonia-lyase defects cause histidinemia which is characterized by increased histidine and histamine and decreased urocanic acid in body fluids

References

1.Increased Sensitivity of Histidinemic Mice to UVB Radiation Suggests a Crucial Role of Endogenous Urocanic Acid in Photoprotection.Barresi C, Stremnitzer C, Mlitz V, Kezic S, Kammeyer A, Ghannadan M, Posa-Markaryan K, Selden C, Tschachler E, Eckhart L.J Invest Dermatol. 2010 Aug 5. [Epub ahead of print]2.Histidase expression in human epidermal keratinocytes: Regulation by differentiation status and all-trans retinoic acid.Eckhart L, Schmidt M, Mildner M, Mlitz V, Abtin A, Ballaun C, Fischer H, Mrass P, Tschachler E.J Dermatol Sci. 2008 Jun;50(3):209-15. Epub 2008 Feb 15.

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$ 350.00
Cat# AT2311a
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Availability: 7-10 days
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