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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   HARS Antibody (monoclonal) (M01)   

HARS Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant HARS.

  • WB - HARS Antibody (monoclonal) (M01) AT2317a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.3 KDa) .
  • WB - HARS Antibody (monoclonal) (M01) AT2317a
    HARS monoclonal antibody (M01), clone 1C8 Western Blot analysis of HARS expression in HeLa ( (Cat # AT2317a )
  • IF - HARS Antibody (monoclonal) (M01) AT2317a
    Immunofluorescence of monoclonal antibody to HARS on HeLa cell. [antibody concentration 10 ug/ml]
  • E - HARS Antibody (monoclonal) (M01) AT2317a
    Detection limit for recombinant GST tagged HARS is approximately 0.03ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P12081
Other Accession NM_002109
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1C8
Calculated MW 57411 Da
Additional Information
Gene ID 3035
Other Names Histidine--tRNA ligase, cytoplasmic, Histidyl-tRNA synthetase, HisRS, HARS, HRS
Target/Specificity HARS (NP_002100, 1 a.a. ~ 96 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsHARS Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.


Clinical significance of anti-histidyl-tRNA synthetase (Jo1) autoantibodies. Gomard-Mennesson E, et al. Ann N Y Acad Sci, 2007 Aug. PMID 17785330. Novel conformation of histidyl-transfer RNA synthetase in the lung: the target tissue in Jo-1 autoantibody-associated myositis. Levine SM, et al. Arthritis Rheum, 2007 Aug. PMID 17665459. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334. Complete sequencing and characterization of 21,243 full-length human cDNAs. Ota T, et al. Nat Genet, 2004 Jan. PMID 14702039. TSG101 interaction with HRS mediates endosomal trafficking and receptor down-regulation. Lu Q, et al. Proc Natl Acad Sci U S A, 2003 Jun 24. PMID 12802020.

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$ 315.00
Cat# AT2317a
(40 western blots)
Availability: 7-10 days
Bulk Size
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