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>   home   >   Products   >   Primary Antibodies   >   HMGB1 Antibody (monoclonal) (M08)   

HMGB1 Antibody (monoclonal) (M08)

Mouse monoclonal antibody raised against a partial recombinant HMGB1.

  • WB - HMGB1 Antibody (monoclonal) (M08) AT2385a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (35.64 KDa) .
  • WB - HMGB1 Antibody (monoclonal) (M08) AT2385a
    HMGB1 monoclonal antibody (M08), clone 2F6 Western Blot analysis of HMGB1 expression in Hela S3 NE ( (Cat # AT2385a )
  • WB - HMGB1 Antibody (monoclonal) (M08) AT2385a
    Western Blot analysis of HMGB1 expression in transfected 293T cell line by HMGB1 monoclonal antibody (M08), clone 2F6.

    Lane 1: HMGB1 transfected lysate(25 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - HMGB1 Antibody (monoclonal) (M08) AT2385a
    Immunoperoxidase of monoclonal antibody to HMGB1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
  • IF - HMGB1 Antibody (monoclonal) (M08) AT2385a
    Immunofluorescence of monoclonal antibody to HMGB1 on HeLa cell. [antibody concentration 10 ug/ml]
  • E - HMGB1 Antibody (monoclonal) (M08) AT2385a
    Detection limit for recombinant GST tagged HMGB1 is approximately 0.3ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P09429
Other Accession NM_002128
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 2F6
Calculated MW 24894 Da
Additional Information
Gene ID 3146
Other Names High mobility group protein B1, High mobility group protein 1, HMG-1, HMGB1, HMG1
Target/Specificity HMGB1 (NP_002119, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsHMGB1 Antibody (monoclonal) (M08) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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1.The Anti-Inflammatory Activity of HMGB1 A Box Is Enhanced When Fused with C-Terminal Acidic Tail.Gong W, Zheng Y, Chao F, Li Y, Xu Z, Huang G, Gao X, Li S, He F.J Biomed Biotechnol. 2010;2010:915234. Epub 2010 Apr 1.2.Functional dissection of an IFN-alpha/beta receptor 1 promoter variant that confers higher risk to chronic hepatitis B virus infection.Zhou J, Huang JD, Poon VK, Chen DQ, Chan CC, Ng F, Guan XY, Watt RM, Lu L, Yuen KY, Zheng BJ.J Hepatol. 2009 Aug;51(2):322-32. Epub 2009 May 3.

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$ 315.00
Cat# AT2385a
(40 western blots)
Availability: 7-10 days
Bulk Size
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