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HOXB5 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant HOXB5.

     
  • WB - HOXB5 Antibody (monoclonal) (M01) AT2410a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.52 KDa) .
    detail
  • WB - HOXB5 Antibody (monoclonal) (M01) AT2410a
    Western Blot analysis of HOXB5 expression in transfected 293T cell line by HOXB5 monoclonal antibody (M01), clone 3F10.

    Lane 1: HOXB5 transfected lysate(29.4 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - HOXB5 Antibody (monoclonal) (M01) AT2410a
    Detection limit for recombinant GST tagged HOXB5 is approximately 0.1ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P09067
Other Accession NM_002147
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3F10
Calculated MW 29434 Da
Additional Information
Gene ID 3215
Other Names Homeobox protein Hox-B5, Homeobox protein HHOC10, Homeobox protein Hox-2A, Homeobox protein Hu-1, HOXB5, HOX2A
Target/Specificity HOXB5 (NP_002138.1, 170 a.a. ~ 267 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsHOXB5 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)
citation

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Background

This gene is a member of the Antp homeobox family and encodes a nuclear protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17. The encoded protein functions as a sequence-specific transcription factor that is involved in lung and gut development. Increased expression of this gene is associated with a distinct biologic subset of acute myeloid leukemia (AML) and the occurrence of bronchopulmonary sequestration (BPS) and congenital cystic adenomatoid malformation (CCAM) tissue.

References

DNA methylation profiling of ovarian carcinomas and their in vitro models identifies HOXA9, HOXB5, SCGB3A1, and CRABP1 as novel targets. Wu Q, et al. Mol Cancer, 2007 Jul 10. PMID 17623056.The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.Expression of Hoxb-5 during human lung development and in congenital lung malformations. Volpe MV, et al. Birth Defects Res A Clin Mol Teratol, 2003 Aug. PMID 14632303.HOXB5 expression is spatially and temporarily regulated in human embryonic gut during neural crest cell colonization and differentiation of enteric neuroblasts. Fu M, et al. Dev Dyn, 2003 Sep. PMID 12950074.HoxB5 is an upstream transcriptional switch for differentiation of the vascular endothelium from precursor cells. Wu Y, et al. Mol Cell Biol, 2003 Aug. PMID 12897140.

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$ 350.00
Cat# AT2410a
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Availability: 7-10 days
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