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ID1 Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a full length recombinant ID1.

  • WB - ID1 Antibody (monoclonal) (M02) AT2473a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (42.79 KDa) .
  • WB - ID1 Antibody (monoclonal) (M02) AT2473a
    Western Blot analysis of ID1 expression in transfected 293T cell line by ID1 monoclonal antibody (M02), clone 1F7.

    Lane 1: ID1 transfected lysate(16.1 KDa).
    Lane 2: Non-transfected lysate.
  • IF - ID1 Antibody (monoclonal) (M02) AT2473a
    Immunofluorescence of monoclonal antibody to ID1 on HeLa cell. [antibody concentration 10 ug/ml]
  • E - ID1 Antibody (monoclonal) (M02) AT2473a
    Detection limit for recombinant GST tagged ID1 is approximately 0.1ng/ml as a capture antibody.
  • WB - ID1 Antibody (monoclonal) (M02) AT2473a
    Western blot analysis of ID1 over-expressed 293 cell line, cotransfected with ID1 Validated Chimera RNAi ( (Cat # AT2473a )
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P41134
Other Accession BC000613
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1F7
Calculated MW 16133 Da
Additional Information
Gene ID 3397
Other Names DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24, bHLHb24, Inhibitor of DNA binding 1, Inhibitor of differentiation 1, ID1, BHLHB24, ID
Target/Specificity ID1 (AAH00613.1, 1 a.a. ~ 155 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsID1 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene.


Smad-dependent and smad-independent induction of id1 by prostacyclin analogues inhibits proliferation of pulmonary artery smooth muscle cells in vitro and in vivo. Yang J, et al. Circ Res, 2010 Jul 23. PMID 20522807.Upregulation of SOX2, NOTCH1, and ID1 in supratentorial primitive neuroectodermal tumors: a distinct differentiation pattern from that of medulloblastomas. Phi JH, et al. J Neurosurg Pediatr, 2010 Jun. PMID 20515335.ID1 enhances docetaxel cytotoxicity in prostate cancer cells through inhibition of p21. Geng H, et al. Cancer Res, 2010 Apr 15. PMID 20388787.The core binding factor CBF negatively regulates skeletal muscle terminal differentiation. Philipot O, et al. PLoS One, 2010 Feb 25. PMID 20195544.Inhibitor of differentiation 1 (ID1) promotes cell survival and proliferation of prostate epithelial cells. Schmidt M, et al. Cell Mol Biol Lett, 2010 Jun. PMID 20186495.

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$ 315.00
Cat# AT2473a
(40 western blots)
Availability: 7-10 days
Bulk Size
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