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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   IKBKG Antibody (monoclonal) (M01)   

IKBKG Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant IKBKG.

  • WB - IKBKG Antibody (monoclonal) (M01) AT2499a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (71.83 KDa) .
  • WB - IKBKG Antibody (monoclonal) (M01) AT2499a
    IKBKG monoclonal antibody (M01), clone 4G9 Western Blot analysis of IKBKG expression in K-562 ( (Cat # AT2499a )
  • IHC - IKBKG Antibody (monoclonal) (M01) AT2499a
    Immunoperoxidase of monoclonal antibody to IKBKG on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]
  • IF - IKBKG Antibody (monoclonal) (M01) AT2499a
    Immunofluorescence of monoclonal antibody to IKBKG on HeLa cell. [antibody concentration 8 ug/ml]
  • E - IKBKG Antibody (monoclonal) (M01) AT2499a
    Detection limit for recombinant GST tagged IKBKG is approximately 0.1ng/ml as a capture antibody.
  •  - IKBKG Antibody (monoclonal) (M01) AT2499a
    Proximity Ligation Analysis of protein-protein interactions between RALBP1 and IKBKG HeLa cells were stained with anti-RALBP1 rabbit purified polyclonal 1:1200 and anti-IKBKG mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y6K9
Other Accession BC050612
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 4G9
Calculated MW 48198 Da
Additional Information
Gene ID 8517
Other Names NF-kappa-B essential modulator, NEMO, FIP-3, IkB kinase-associated protein 1, IKKAP1, Inhibitor of nuclear factor kappa-B kinase subunit gamma, I-kappa-B kinase subunit gamma, IKK-gamma, IKKG, IkB kinase subunit gamma, NF-kappa-B essential modifier, IKBKG, FIP3, NEMO
Target/Specificity IKBKG (AAH50612, 1 a.a. ~ 419 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsIKBKG Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome.


Immune deficiency caused by impaired expression of nuclear factor-kappaB essential modifier (NEMO) because of a mutation in the 5' untranslated region of the NEMO gene. Mooster JL, et al. J Allergy Clin Immunol, 2010 Jul. PMID 20542322.NEMO gene mutations in Chinese patients with incontinentia pigmenti. Hsiao PF, et al. J Formos Med Assoc, 2010 Mar. PMID 20434027.The LCR at the IKBKG locus is prone to recombine. Fusco F, et al. Am J Hum Genet, 2010 Apr 9. PMID 20380930.IKK{gamma} protein is a target of BAG3 regulatory activity in human tumor growth. Ammirante M, et al. Proc Natl Acad Sci U S A, 2010 Apr 20. PMID 20368414.Activation of noncanonical NF-kappaB signaling by the oncoprotein Tio. de Jong SJ, et al. J Biol Chem, 2010 May 28. PMID 20353939.

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$ 315.00
Cat# AT2499a
(40 western blots)
Availability: 7-10 days
Bulk Size
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