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IRX3 Antibody (monoclonal) (M03)

Mouse monoclonal antibody raised against a partial recombinant IRX3.

     
  • IF - IRX3 Antibody (monoclonal) (M03) AT2550a
    Immunofluorescence of monoclonal antibody to IRX3 on HeLa cell. [antibody concentration 30 ug/ml]
    detail
  • WB - IRX3 Antibody (monoclonal) (M03) AT2550a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.18 KDa) .
    detail
  • WB - IRX3 Antibody (monoclonal) (M03) AT2550a
    IRX3 monoclonal antibody (M03), clone 3D8. Western Blot analysis of IRX3 expression in Hela S3 NE ( (Cat # AT2550a )
    detail
  • WB - IRX3 Antibody (monoclonal) (M03) AT2550a
    IRX3 monoclonal antibody (M03), clone 3D8 Western Blot analysis of IRX3 expression in K-562 ( (Cat # AT2550a )
    detail
  • E - IRX3 Antibody (monoclonal) (M03) AT2550a
    Detection limit for recombinant GST tagged IRX3 is approximately 0.03ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession P78415
Other Accession NM_024336
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3D8
Calculated MW 52119 Da
Additional Information
Gene ID 79191
Other Names Iroquois-class homeodomain protein IRX-3, Homeodomain protein IRXB1, Iroquois homeobox protein 3, IRX3, IRXB1
Target/Specificity IRX3 (NP_077312, 182 a.a. ~ 285 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsIRX3 Antibody (monoclonal) (M03) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

IRX3 is a member of the Iroquois homeobox gene family (see IRX1; MIM 606197) and plays a role in an early step of neural development (Bellefroid et al., 1998 [PubMed 9427753]). Members of this family appear to play multiple roles during pattern formation of vertebrate embryos (Lewis et al., 1999 [PubMed 10370142]).

References

Long-range gene regulation links genomic type 2 diabetes and obesity risk regions to HHEX, SOX4, and IRX3. Ragvin A, et al. Proc Natl Acad Sci U S A, 2010 Jan 12. PMID 20080751.Coeliac disease-associated risk variants in TNFAIP3 and REL implicate altered NF-kappaB signalling. Trynka G, et al. Gut, 2009 Aug. PMID 19240061.The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Strausberg RL, et al. Proc Natl Acad Sci U S A, 2002 Dec 24. PMID 12477932.Regulated expression patterns of IRX-2, an Iroquois-class homeobox gene, in the human breast. Lewis MT, et al. Cell Tissue Res, 1999 Jun. PMID 10370142.

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$ 350.00
Cat# AT2550a
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Availability: 7-10 days
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