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KRAS Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant KRAS.

     
  • IF - KRAS Antibody (monoclonal) (M01) AT2650a
    Immunofluorescence of monoclonal antibody to KRAS on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - KRAS Antibody (monoclonal) (M01) AT2650a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (46.42 KDa) .
    detail
  • WB - KRAS Antibody (monoclonal) (M01) AT2650a
    KRAS monoclonal antibody (M01), clone 3B10-2F2 Western Blot analysis of KRAS expression in HeLa ( (Cat # AT2650a )
    detail
  • WB - KRAS Antibody (monoclonal) (M01) AT2650a
    Western Blot analysis of KRAS expression in transfected 293T cell line by KRAS monoclonal antibody (M01), clone 3B10-2F2.

    Lane 1: KRAS transfected lysate(21 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - KRAS Antibody (monoclonal) (M01) AT2650a
    Detection limit for recombinant GST tagged KRAS is approximately 1ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS: 1
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession P01116
Other Accession BC013572
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names S2
Calculated MW 21656 Da
Additional Information
Gene ID 3845
Other Names GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, GTPase KRas, N-terminally processed, KRAS, KRAS2, RASK2
Target/Specificity KRAS (AAH13572, 1 a.a. ~ 188 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsKRAS Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations ( 0 )

Background

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

References

1.Evidence for aldosterone-dependent growth of renal cell carcinoma.King S, Bray S, Galbraith S, Christie L, Fleming SInt J Exp Pathol. 2014 May 7. doi: 10.1111/iep.12074.2.KRAS gene amplification in colorectal cancer and impact on response to EGFR-targeted therapy.Valtorta E, Misale S, Sartore-Bianchi A, Nagtegaal ID, Paraf F, Lauricella C, Dimartino V, Hobor S, Jacobs B, Ercolani C, Lamba S, Scala E, Veronese S, Laurent-Puig P, Siena S, Tejpar S, Mottolese M, Punt CJ, Gambacorta M, Bardelli A, Di Nicolantonio FInt J Cancer. 2013 Feb 12. doi: 10.1002/ijc.28106.3.Analysis of k-ras nuclear expression in fibroblasts and mesangial cells.Fuentes-Calvo I, Blazquez-Medela AM, Santos E, Lopez-Novoa JM, Martinez-Salgado C.PLoS One. 2010 Jan 14;5(1):e8703.

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$ 350.00
Cat# AT2650a
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Availability: 7-10 days
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