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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   LDB3 Antibody (monoclonal) (M06)   

LDB3 Antibody (monoclonal) (M06)

Mouse monoclonal antibody raised against a full-length recombinant LDB3.

  • WB - LDB3 Antibody (monoclonal) (M06) AT2686a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (56.87 KDa) .
  • WB - LDB3 Antibody (monoclonal) (M06) AT2686a
    LDB3 monoclonal antibody (M06), clone 3C8 Western Blot analysis of LDB3 expression in A-431 ( (Cat # AT2686a )
  • WB - LDB3 Antibody (monoclonal) (M06) AT2686a
    Western Blot analysis of LDB3 expression in transfected 293T cell line by LDB3 monoclonal antibody (M06), clone 3C8.

    Lane 1: LDB3 transfected lysate(31 KDa).
    Lane 2: Non-transfected lysate.
  • IF - LDB3 Antibody (monoclonal) (M06) AT2686a
    Immunofluorescence of monoclonal antibody to LDB3 on A-431 cell . [antibody concentration 10 ug/ml]
  • E - LDB3 Antibody (monoclonal) (M06) AT2686a
    Detection limit for recombinant GST tagged LDB3 is approximately 0.03ng/ml as a capture antibody.
  • WB - LDB3 Antibody (monoclonal) (M06) AT2686a
    Western blot analysis of LDB3 over-expressed 293 cell line, cotransfected with LDB3 Validated Chimera RNAi ( (Cat # AT2686a )
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O75112
Other Accession BC010929
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names 3C8
Calculated MW 77135 Da
Additional Information
Gene ID 11155
Other Names LIM domain-binding protein 3, Protein cypher, Z-band alternatively spliced PDZ-motif protein, LDB3 (HGNC:15710)
Target/Specificity LDB3 (AAH10929, 1 a.a. ~ 283 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsLDB3 Antibody (monoclonal) (M06) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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This gene encodes a PDZ domain-containing protein. PDZ motifs are modular protein-protein interaction domains consisting of 80-120 amino acid residues. PDZ domain-containing proteins interact with each other in cytoskeletal assembly or with other proteins involved in targeting and clustering of membrane proteins. The protein encoded by this gene interacts with alpha-actinin-2 through its N-terminal PDZ domain and with protein kinase C via its C-terminal LIM domains. The LIM domain is a cysteine-rich motif defined by 50-60 amino acids containing two zinc-binding modules. This protein also interacts with all three members of the myozenin family. Mutations in this gene have been associated with myofibrillar myopathy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been identified; all isoforms have N-terminal PDZ domains while only longer isoforms (1, 2 and 5) have C-terminal LIM domains.


1.Z-disc-associated, Alternatively Spliced, PDZ Motif-containing Protein (ZASP) Mutations in the Actin-binding Domain Cause Disruption of Skeletal Muscle Actin Filaments in Myofibrillar Myopathy.Lin X, Ruiz J, Bajraktari I, Ohman R, Banerjee S, Gribble K, Kaufman JD, Wingfield PT, Griggs RC, Fischbeck KH, Mankodi AJ Biol Chem. 2014 May 9;289(19):13615-26. doi: 10.1074/jbc.M114.550418. Epub 2014 Mar 25.2.Impaired binding of ZASP/Cypher with phosphoglucomutase 1 is associated with dilated cardiomyopathy.Arimura T, Inagaki N, Hayashi T, Shichi D, Sato A, Hinohara K, Vatta M, Towbin JA, Chikamori T, Yamashina A, Kimura A.Cardiovasc Res. 2009 Jul 1;83(1):80-8. Epub 2009 Apr 17.

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$ 315.00
Cat# AT2686a
Availability: 7-10 days
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