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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   LOC51255 Antibody (monoclonal) (M01)   

LOC51255 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant RNF181.

  • WB - LOC51255 Antibody (monoclonal) (M01) AT2734a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (32.78 KDa) .
  • WB - LOC51255 Antibody (monoclonal) (M01) AT2734a
    RNF181 monoclonal antibody (M01), clone 5A7 Western Blot analysis of RNF181 expression in Jurkat ( (Cat # AT2734a )
  • WB - LOC51255 Antibody (monoclonal) (M01) AT2734a
    Western Blot analysis of RNF181 expression in transfected 293T cell line by RNF181 monoclonal antibody (M01), clone 5A7.

    Lane 1: RNF181 transfected lysate(17.9 KDa).
    Lane 2: Non-transfected lysate.
  • IP - LOC51255 Antibody (monoclonal) (M01) AT2734a
    Immunoprecipitation of RNF181 transfected lysate using anti-RNF181 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RNF181 monoclonal antibody.
  • E - LOC51255 Antibody (monoclonal) (M01) AT2734a
    Detection limit for recombinant GST tagged RNF181 is approximately 0.1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9P0P0
Other Accession NM_016494
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 5A7
Calculated MW 17909 Da
Additional Information
Gene ID 51255
Other Names E3 ubiquitin-protein ligase RNF181, 632-, RING finger protein 181, RNF181
Target/Specificity RNF181 (NP_057578, 90 a.a. ~ 153 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsLOC51255 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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RNF181 binds the integrin alpha-IIb (ITGA2B; MIM 607759)/beta-3 (ITGB3; MIM 173470) complex and has E3 ubiquitin ligase activity (Brophy et al., 2008 [PubMed 18331836]).


[Construction of recombinant eukaryotic expression vector pDsRed1-C3/LOC51255 and subcellular localization of LOC51255 protein in HePG2 cell line] Huang X, et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi, 2009 May. PMID 19426595.RN181, a novel ubiquitin E3 ligase that interacts with the KVGFFKR motif of platelet integrin alpha(IIb)beta3. Brophy TM, et al. Biochem Biophys Res Commun, 2008 May 16. PMID 18331836.Identification of intrahepatic cholangiocarcinoma related genes by comparison with normal liver tissues using expressed sequence tags. Wang AG, et al. Biochem Biophys Res Commun, 2006 Jul 7. PMID 16712791.ICln, a novel integrin alphaIIbbeta3-associated protein, functionally regulates platelet activation. Larkin D, et al. J Biol Chem, 2004 Jun 25. PMID 15075326.Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Strausberg RL, et al. Proc Natl Acad Sci U S A, 2002 Dec 24. PMID 12477932.

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$ 315.00
Cat# AT2734a
(40 western blots)
Availability: 7-10 days
Bulk Size
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