MARCH7 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant MARCH7.
|Application ||WB, IF, E|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||78051 Da|
|Other Names||E3 ubiquitin-protein ligase MARCH7, 632-, Axotrophin, Membrane-associated RING finger protein 7, Membrane-associated RING-CH protein VII, MARCH-VII, RING finger protein 177, MARCH7, AXOT, RNF177|
|Target/Specificity||MARCH7 (NP_073737, 66 a.a. ~ 136 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Format||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.|
|Precautions||MARCH7 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
MARCH7 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 22.214.171.124). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Bartee et al., 2004 [PubMed 14722266]).
The E3 ligase axotrophin/MARCH-7: protein expression profiling of human tissues reveals links to adult stem cells. Szigyarto CA, et al. J Histochem Cytochem, 2010 Apr. PMID 19901269.The ubiquitin E3 ligase MARCH7 is differentially regulated by the deubiquitylating enzymes USP7 and USP9X. Nathan JA, et al. Traffic, 2008 Jul. PMID 18410486.Evidence for functional inter-relationships between FOXP3, leukaemia inhibitory factor, and axotrophin/MARCH-7 in transplantation tolerance. Muthukumarana PA, et al. Int Immunopharmacol, 2006 Dec 20. PMID 17161353.E2-25K mediates US11-triggered retro-translocation of MHC class I heavy chains in a permeabilized cell system. Flierman D, et al. Proc Natl Acad Sci U S A, 2006 Aug 1. PMID 16868077.A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. Lim J, et al. Cell, 2006 May 19. PMID 16713569.
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