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>   home   >   Products   >   Primary Antibodies   >   MCM3 Antibody (monoclonal) (M03)   

MCM3 Antibody (monoclonal) (M03)

Mouse monoclonal antibody raised against a partial recombinant MCM3.

  • WB - MCM3 Antibody (monoclonal) (M03) AT2820a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.84 KDa) .
  • WB - MCM3 Antibody (monoclonal) (M03) AT2820a
    MCM3 monoclonal antibody (M03), clone 3E1 Western Blot analysis of MCM3 expression in HeLa ( (Cat # AT2820a )
  • WB - MCM3 Antibody (monoclonal) (M03) AT2820a
    MCM3 monoclonal antibody (M03), clone 3E1. Western Blot analysis of MCM3 expression in Hela S3 NE ( (Cat # AT2820a )
  • IF - MCM3 Antibody (monoclonal) (M03) AT2820a
    Immunofluorescence of monoclonal antibody to MCM3 on HeLa cell. [antibody concentration 10 ug/ml]
  • E - MCM3 Antibody (monoclonal) (M03) AT2820a
    Detection limit for recombinant GST tagged MCM3 is approximately 0.1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P25205
Other Accession NM_002388
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names 30
Calculated MW 90981 Da
Additional Information
Gene ID 4172
Other Names DNA replication licensing factor MCM3, DNA polymerase alpha holoenzyme-associated protein P1, P1-MCM3, RLF subunit beta, p102, MCM3
Target/Specificity MCM3 (NP_002379, 699 a.a. ~ 808 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsMCM3 Antibody (monoclonal) (M03) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.


Human cytomegalovirus IE86 protein binds to cellular Mcm3 protein but does not inhibit its binding to the Epstein-Barr virus oriP in U373MG-p220.2 cells. Song YJ, et al. Acta Virol, 2010. PMID 20545442.Centrosome-related genes, genetic variation, and risk of breast cancer. Olson JE, et al. Breast Cancer Res Treat, 2010 May 28. PMID 20508983.Analysis of interaction partners of H4 histone by a new proteomics approach. Saade E, et al. Proteomics, 2009 Nov. PMID 19862764.Minichromosome maintenance protein 3 is a candidate proliferation marker in papillary thyroid carcinoma. Lee YS, et al. Exp Mol Pathol, 2010 Feb. PMID 19818763.Phosphorylation of MCM3 on Ser-112 regulates its incorporation into the MCM2-7 complex. Lin DI, et al. Proc Natl Acad Sci U S A, 2008 Jun 10. PMID 18524952.

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$ 315.00
Cat# AT2820a
(40 western blots)
Availability: 7-10 days
Bulk Size
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