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MDFI Antibody (monoclonal) (M07)

Mouse monoclonal antibody raised against a partial recombinant MDFI.

  • WB - MDFI Antibody (monoclonal) (M07) AT2824a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (34.32 KDa) .
  • WB - MDFI Antibody (monoclonal) (M07) AT2824a
    Western Blot analysis of MDFI expression in transfected 293T cell line by MDFI monoclonal antibody (M07), clone 3B4.

    Lane 1: MDFI transfected lysate(25.029 KDa).
    Lane 2: Non-transfected lysate.
  • IF - MDFI Antibody (monoclonal) (M07) AT2824a
    Immunofluorescence of monoclonal antibody to MDFI on HeLa cell . [antibody concentration 10 ug/ml]
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q99750
Other Accession NM_005586
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3B4
Calculated MW 25029 Da
Additional Information
Gene ID 4188
Other Names MyoD family inhibitor, Myogenic repressor I-mf, MDFI
Target/Specificity MDFI (NP_005577, 33 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsMDFI Antibody (monoclonal) (M07) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures.


Shifted Transversal Design smart-pooling for high coverage interactome mapping. Xin X, et al. Genome Res, 2009 Jul. PMID 19447967.An empirical framework for binary interactome mapping. Venkatesan K, et al. Nat Methods, 2009 Jan. PMID 19060904.Developmental regulators containing the I-mfa domain interact with T cyclins and Tat and modulate transcription. Wang Q, et al. J Mol Biol, 2007 Mar 30. PMID 17289077.A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. Lim J, et al. Cell, 2006 May 19. PMID 16713569.Towards a proteome-scale map of the human protein-protein interaction network. Rual JF, et al. Nature, 2005 Oct 20. PMID 16189514.

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$ 315.00
Cat# AT2824a
(40 western blots)
Availability: 7-10 days
Bulk Size
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