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NDN Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a partial recombinant NDN.

     
  • IF - NDN Antibody (monoclonal) (M02) AT2988a
    Immunofluorescence of monoclonal antibody to NDN on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - NDN Antibody (monoclonal) (M02) AT2988a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.11 KDa) .
    detail
  • WB - NDN Antibody (monoclonal) (M02) AT2988a
    NDN monoclonal antibody (M02), clone 1B3 Western Blot analysis of NDN expression in HL-60 ( (Cat # AT2988a )
    detail
  • WB - NDN Antibody (monoclonal) (M02) AT2988a
    Western Blot analysis of NDN expression in transfected 293T cell line by NDN monoclonal antibody (M02), clone 1B3.

    Lane 1: NDN transfected lysate(36.1 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • WB - NDN Antibody (monoclonal) (M02) AT2988a
    Western blot analysis of NDN over-expressed 293 cell line, cotransfected with NDN Validated Chimera RNAi ( (Cat # AT2988a )
    detail
  • IHC - NDN Antibody (monoclonal) (M02) AT2988a
    Immunoperoxidase of monoclonal antibody to NDN on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml]
    detail
  • E - NDN Antibody (monoclonal) (M02) AT2988a
    Detection limit for recombinant GST tagged NDN is approximately 0.03ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, E
Primary Accession Q99608
Other Accession NM_002487
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1B3
Calculated MW 36086 Da
Additional Information
Gene ID 4692
Other Names Necdin, NDN
Target/Specificity NDN (NP_002478, 222 a.a. ~ 321 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsNDN Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This intronless gene is located in the Prader-Willi syndrome deletion region. It is an imprinted gene and is expressed exclusively from the paternal allele. Studies in mouse suggest that the protein encoded by this gene may suppress growth in postmitotic neurons.

References

1.Necdin Enhances Myoblasts Survival by Facilitating the Degradation of the Mediator of Apoptosis CCAR1/CARP1.Francois S, D'Orlando C, Fatone T, Touvier T, Pessina P, Meneveri R, Brunelli S.PLoS One. 2012;7(8):e43335. Epub 2012 Aug 14.

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$ 350.00
Cat# AT2988a
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