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NEDD1 Antibody (monoclonal) (M05)

Mouse monoclonal antibody raised against a partial recombinant NEDD1.

  • WB - NEDD1 Antibody (monoclonal) (M05) AT3009a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
  • IHC - NEDD1 Antibody (monoclonal) (M05) AT3009a
    Immunoperoxidase of monoclonal antibody to NEDD1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.75 ug/ml]
  • E - NEDD1 Antibody (monoclonal) (M05) AT3009a
    Detection limit for recombinant GST tagged NEDD1 is approximately 0.03ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8NHV4
Other Accession NM_152905
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2b Kappa
Clone Names 7D10
Calculated MW 71966 Da
Additional Information
Gene ID 121441
Other Names Protein NEDD1, Neural precursor cell expressed developmentally down-regulated protein 1, NEDD-1, NEDD1
Target/Specificity NEDD1 (NP_690869, 561 a.a. ~ 660 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsNEDD1 Antibody (monoclonal) (M05) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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1.The dynamics of microtubule minus ends in the human mitotic spindle.Lecland N, Luders J.Nat Cell Biol. 2014 Jun 29. doi: 10.1038/ncb2996.2.Functional central spindle assembly requires de novo microtubule generation in the interchromosomal region during anaphase.Uehara R, Goshima G.J Cell Biol. 2010 Oct 11. [Epub ahead of print]3.The {gamma}TuRC Revisited: A Comparative Analysis of Interphase and Mitotic Human {gamma}TuRC Redefines the Set of Core Components and Identifies the Novel Subunit GCP8.Teixido-Travesa N, Villen J, Lacasa C, Bertran MT, ArchintiM, Gygi SP, Caelles C, Roig J, Luders J.Originally published as MBoC in Press, 10.1091/mbc.E10-05-04084.SPICE - a previously uncharacterized protein required for centriole duplication and mitotic chromosome congression.Archinti M, Lacasa C, Teixido-Travesa N, Luders J.J Cell Sci. 2010 Sep 15;123(Pt 18):3039-46. Epub 2010 Aug 24.5.Axon Extension Occurs Independently of Centrosomal Microtubule Nucleation.Stiess M, Maghelli N, Kapitein LC, Gomis-Ruth S, Wilsch-Brauninger M, Hoogenraad CC, Tolic-Norrelykke IM, Bradke F.Science. 2010 Feb 5;327(5966):704-7. Epub 2010 Jan 7.6.FAM29A, a target of Plk1 regulation, controls the partitioning of NEDD1 between the mitotic spindle and the centrosomes.Zhu H, Fang K, Fang G.J Cell Sci. 2009 Aug 1;122(Pt 15):2750-9. Epub 2009 Jul 13.7.Plk1-dependent recruitment of gamma-tubulin complexes to mitotic centrosomes involves multiple PCM components.Haren L, Stearns T, Luders J.PLoS One. 2009 Jun 19;4(6):e5976.8.FAM29A promotes microtubule amplification via recruitment of the NEDD1-gamma-tubulin complex to the mitotic spindle.Zhu H, Coppinger JA, Jang CY, Yates JR, III, Fang J.J Cell Biol. 2008 Dec 1;183(5):835-48. Epub 2008 Nov 24.

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$ 315.00
Cat# AT3009a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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