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NEK3 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant NEK3.
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF, E |
|---|---|
| Primary Accession | P51956 |
| Other Accession | BC019916 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 Kappa |
| Clone Names | 2F8 |
| Calculated MW | 57705 Da |
| Gene ID | 4752 |
|---|---|
| Other Names | Serine/threonine-protein kinase Nek3, HSPK 36, Never in mitosis A-related kinase 3, NimA-related protein kinase 3, NEK3 |
| Target/Specificity | NEK3 (AAH19916, 406 a.a. ~ 506 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | NEK3 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene.
References
Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.Nek3 kinase regulates prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells. Miller SL, et al. Oncogene, 2007 Jul 12. PMID 17297458.Is there any association between nek3 and cancers with frequent 13q14 deletion? Hern?ndez M, et al. Cancer Invest, 2006 Nov. PMID 17118778.Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Kimura K, et al. Genome Res, 2006 Jan. PMID 16344560.Novel association of Vav2 and Nek3 modulates signaling through the human prolactin receptor. Miller SL, et al. Mol Endocrinol, 2005 Apr. PMID 15618286.
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