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NEK3 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant NEK3.

  • WB - NEK3 Antibody (monoclonal) (M01) AT3017a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
  • WB - NEK3 Antibody (monoclonal) (M01) AT3017a
    Western Blot analysis of NEK3 expression in transfected 293T cell line by NEK3 monoclonal antibody (M01), clone 2F8.

    Lane 1: NEK3 transfected lysate(57.7 KDa).
    Lane 2: Non-transfected lysate.
  • IF - NEK3 Antibody (monoclonal) (M01) AT3017a
    Immunofluorescence of monoclonal antibody to NEK3 on HeLa cell. [antibody concentration 10 ug/ml]
  • E - NEK3 Antibody (monoclonal) (M01) AT3017a
    Detection limit for recombinant GST tagged NEK3 is approximately 0.03ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P51956
Other Accession BC019916
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 2F8
Calculated MW 57705 Da
Additional Information
Gene ID 4752
Other Names Serine/threonine-protein kinase Nek3, HSPK 36, Never in mitosis A-related kinase 3, NimA-related protein kinase 3, NEK3
Target/Specificity NEK3 (AAH19916, 406 a.a. ~ 506 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsNEK3 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations ( 0 )


This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene.


Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.Nek3 kinase regulates prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells. Miller SL, et al. Oncogene, 2007 Jul 12. PMID 17297458.Is there any association between nek3 and cancers with frequent 13q14 deletion? Hern?ndez M, et al. Cancer Invest, 2006 Nov. PMID 17118778.Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Kimura K, et al. Genome Res, 2006 Jan. PMID 16344560.Novel association of Vav2 and Nek3 modulates signaling through the human prolactin receptor. Miller SL, et al. Mol Endocrinol, 2005 Apr. PMID 15618286.

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$ 315.00
Cat# AT3017a
(40 western blots)
Availability: 7-10 days
Bulk Size
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