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>   home   >   Products   >   Primary Antibodies   >   NFE2 Antibody (monoclonal) (M01)   

NFE2 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full-length recombinant NFE2.

     
  • WB - NFE2 Antibody (monoclonal) (M01) AT3029a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (66.77 KDa) .
  • WB - NFE2 Antibody (monoclonal) (M01) AT3029a
    Western Blot analysis of NFE2 expression in transfected 293T cell line by NFE2 monoclonal antibody (M01), clone 2C6.

    Lane 1: NFE2 transfected lysate(41.5 KDa).
    Lane 2: Non-transfected lysate.
  • E - NFE2 Antibody (monoclonal) (M01) AT3029a
    Detection limit for recombinant GST tagged NFE2 is approximately 3ng/ml as a capture antibody.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q16621
Other Accession BC005044
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 2C6
Calculated MW 41473 Da
Additional Information
Gene ID 4778
Other Names Transcription factor NF-E2 45 kDa subunit, Leucine zipper protein NF-E2, Nuclear factor, erythroid-derived 2 45 kDa subunit, p45 NF-E2, NFE2
Target/Specificity NFE2 (AAH05044, 1 a.a. ~ 373 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsNFE2 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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References

AML1 is overexpressed in patients with myeloproliferative neoplasms and mediates JAK2V617F-independent overexpression of NF-E2. Wang W, et al. Blood, 2010 Jul 15. PMID 20339092.USF and NF-E2 cooperate to regulate the recruitment and activity of RNA polymerase II in the beta-globin gene locus. Zhou Z, et al. J Biol Chem, 2010 May 21. PMID 20236933.JNK-mediated turnover and stabilization of the transcription factor p45/NF-E2 during differentiation of murine erythroleukemia cells. Lee TL, et al. Proc Natl Acad Sci U S A, 2010 Jan 5. PMID 19966288.NF-E2 domination over Nrf2 promotes ROS accumulation and megakaryocytic maturation. Motohashi H, et al. Blood, 2010 Jan 21. PMID 19901266.A genome-wide meta-analysis identifies 22 loci associated with eight hematological parameters in the HaemGen consortium. Soranzo N, et al. Nat Genet, 2009 Nov. PMID 19820697.

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$ 315.00
Cat# AT3029a
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